A bleached-kraft mill effluent fraction causing induction of a fish mixed-function oxygenase enzyme
Burnison, BK; Hodson, PV; Nuttley, DJ; Efler, S
| HERO ID | 1190403 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | A bleached-kraft mill effluent fraction causing induction of a fish mixed-function oxygenase enzyme |
| Authors | Burnison, BK; Hodson, PV; Nuttley, DJ; Efler, S |
| Journal | Environmental Toxicology and Chemistry |
| Volume | 15 |
| Issue | 9 |
| Page Numbers | 1524-1531 |
| Abstract | BIOSIS COPYRIGHT: BIOL ABS. Pulp mill effluents contain a myriad of chemicals that have the potential to cause deleterious effects on aquatic biota in receiving waters. Some of these chemicals evoke an acute lethal response of exposed biota while others evoke sublethal responses. One such sublethal response is the induction of mixed-function oxygenases (MFO) in fish, specifically the CYP1A1 enzyme ethoxyresorufin-o-deethylase (EROD). We followed the partitioning of the inducing chemicals in pulp mill effluent fractions by Toxicity Identification Evaluation (TIE), or bioassay-driven chemical analysis. This procedure was eventually modified to a more direct technique involving centrifugation, filtration, cleanup procedures, and C18 solid-phase adsorption. The extracts from the fractionation of two pulp mill effluents after secondary treatment were tested for EROD-inducing activity in a 4-d rainbow trout bioassay. The methanol extracts of particulates/colloids showed significant inducing capacity in |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1996VF25200015 |
| Is Public | Yes |
| Language Text | English |
| Keyword | pulp mill effluents; mixed-function oxygenase; EROD; HPLC fractionation; dissolved organic carbon |
| Is Qa | No |