[Comparison of estrogenic activity of phthalate esters in vitro]
Yang, X; Yi, QH; Zhou, QQ; Mu, W; Zhang, C; He, Z; Hu, XJ; Xiong, L; Liu, DL
HERO ID
1249555
Reference Type
Journal Article
Year
2011
Language
Chinese
| HERO ID | 1249555 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | [Comparison of estrogenic activity of phthalate esters in vitro] |
| Authors | Yang, X; Yi, QH; Zhou, QQ; Mu, W; Zhang, C; He, Z; Hu, XJ; Xiong, L; Liu, DL |
| Journal | Huanjing Kexue yu Jiazhu / Environmental Science & Technology |
| Volume | 34 |
| Issue | 3 (Mar 2011) |
| Page Numbers | 1-5 |
| Abstract | A yeast-based assay was used to estimate the estrogenic potential of phthalate esters. The yeast transformants contained human-estrogen receptor alpha gene along with the estrogenic responsive elements upstream of beta -galactosidase reporter gene. Five phthalate esters were added to yeast cultures in doses ranging from 1.0x10(-3)~1.0x10(-11) mol/L and following incubation, the yeasts were then lysed and assayed for beta -galactosidase activity. Relative potency ratios were calculated and used to compare the estrogenic activity of five phthalate esters along with beta -galactosidase activity. Results showed that diethyl phthalate (DEP, 1x10(-7)~1x10(-9) mol/L), dibutyl phthalate (DBP, 1x10(-6)~1x10(-9) mol/L and BBP, 1x10(-3)~1x10(-6) mol/L show obviously estrogenic activity while dimethyl phthalate (DMP) and di-sec-octyl phthalate (DOP) do not. Relative potency ratios of DEP, DBP and BBP were 8.85x10(-2), 2.54x10(-2) and 8.82x10(-6) respectively, suggesting that DEP and DBP showed higher estrogenic activity than BBP. However, the maximum beta -galactosidase activity induced by BBP was the same as DEP, but higher than DBP. Although the classical nuclear estrogen receptors pathway hypothesis was accepted for decades, other rapid signal transductional pathways have been found recently. The yeast-based assay was only used for primary screening, it is strongly recommended to use other ways to explore estrogenic activity for chemicals. |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Chinese |
| Keyword | Esters; phthalates; Chemicals; Yeasts; upstream; estrogens |