Spray drying from organic solvents to prepare nanoporous/nanoparticulate microparticles of protein: excipient composites designed for oral inhalation
Ní Ógáin, O; Tajber, L; Corrigan, OI; Healy, AM
| HERO ID | 1445007 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Spray drying from organic solvents to prepare nanoporous/nanoparticulate microparticles of protein: excipient composites designed for oral inhalation |
| Authors | Ní Ógáin, O; Tajber, L; Corrigan, OI; Healy, AM |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 64 |
| Issue | 9 |
| Page Numbers | 1275-1290 |
| Abstract | <strong>OBJECTIVES: </strong>The aim of this study was to determine if spray-drying could successfully produce microparticles containing the model protein trypsin in a form suitable for inhalation.<br /><br /><strong>METHODS: </strong>Trypsin was spray-dried with raffinose from a methanol : n-butyl acetate solvent system (MeOH : BA). The solvent system was then adjusted to include water, and trypsin was co-spray-dried with raffinose, trehalose or hydroxpropyl-β-cyclodextrin. The spray-dried products were characterised by SEM, XRD, DSC, TGA and FTIR. Protein biological activity and in-vitro deposition of trypsin : excipient nanoporous/nanoparticulate microparticles (NPMPs) was also assessed.<br /><br /><strong>KEY FINDINGS: </strong>The inclusion of water in a MeOH : BA solvent system allowed for the successful production of NPMPs of trypsin : excipient by spray-drying. Trypsin formulated as trypsin : excipient NPMPs retained biological activity on processing and showed no deterioration in activity or morphological characteristics when stored with desiccant at either 4 or 25°C. Hydroxpropyl-β-cyclodextrin showed advantages over the sugars in terms of producing powders with appropriate density and with greater physical stability under high-humidity conditions. Fine particle fractions of between 41 and 45% were determined for trypsin : excipient NPMPs.<br /><br /><strong>CONCLUSIONS: </strong>NPMPs of trypsin : excipient systems can be produced by spray-drying by adjustment of the solvent system to allow for adequate solubility of trypsin. |
| Doi | 10.1111/j.2042-7158.2012.01488.x |
| Pmid | 22881440 |
| Wosid | WOS:000307217900008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000307217900008 |
| Is Public | Yes |
| Language Text | English |
| Keyword | inhalation; nanoporous; nanoparticulate microparticles; spray-drying; trypsin |