Nesprin interchain associations control nuclear size
Lu, W; Schneider, M; Neumann, S; Jaeger, VM; Taranum, S; Munck, M; Cartwright, S; Richardson, C; Carthew, J; Noh, K; Goldberg, M; Noegel, AA; Karakesisoglou, I
HERO ID
1450053
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1450053 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Nesprin interchain associations control nuclear size |
| Authors | Lu, W; Schneider, M; Neumann, S; Jaeger, VM; Taranum, S; Munck, M; Cartwright, S; Richardson, C; Carthew, J; Noh, K; Goldberg, M; Noegel, AA; Karakesisoglou, I |
| Journal | Cellular and Molecular Life Sciences (CMLS) |
| Volume | 69 |
| Issue | 20 |
| Page Numbers | 3493-3509 |
| Abstract | Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and associates instead to plectin, which binds intermediate filaments. Nesprins are integrated into the outer nuclear membrane via their C-terminal KASH-domain. Here, we show that nesprin-1/-2 ABDs physically and functionally interact with nesprin-3. Thus, both ends of nesprin-1/-2 giant are integrated at the nuclear surface: via the C-terminal KASH-domain and the N-terminal ABD-nesprin-3 association. Interestingly, nesprin-2 ABD or KASH-domain overexpression leads to increased nuclear areas. Conversely, nesprin-2 mini (contains the ABD and KASH-domain but lacks the massive nesprin-2 giant rod segment) expression yields smaller nuclei. Nuclear shrinkage is further enhanced upon nesprin-3 co-expression or microfilament depolymerization. Our findings suggest that multivariate intermolecular nesprin interactions with the cytoskeleton form a lattice-like filamentous network covering the outer nuclear membrane, which determines nuclear size. |
| Doi | 10.1007/s00018-012-1034-1 |
| Pmid | 22653047 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |