Butyrate enhancement of inteleukin-1β production via activation of oxidative stress pathways in lipopolysaccharide-stimulated THP-1 cells
Ohira, H; Fujioka, Y; Katagiri, C; Yano, M; Mamoto, R; Aoyama, M; Usami, M; Ikeda, M
HERO ID
1455044
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1455044 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Butyrate enhancement of inteleukin-1β production via activation of oxidative stress pathways in lipopolysaccharide-stimulated THP-1 cells |
| Authors | Ohira, H; Fujioka, Y; Katagiri, C; Yano, M; Mamoto, R; Aoyama, M; Usami, M; Ikeda, M |
| Journal | Journal of Clinical Biochemistry and Nutrition |
| Volume | 50 |
| Issue | 1 |
| Page Numbers | 59-66 |
| Abstract | In inflammatory bowel diseases, interleukin-1β production is accelerated. Butyrate, a short chain fatty acid, plays an important role in inflammatory bowel diseases. We investigated the effect of butyrate on interleukin-1β production in macrophage and elucidated its underlying mechanism. We stimulated THP-1 cells, a human premonocytic cell line, by lipopolysaccharide alone and by butyrate with lipopolysaccharide. Butyrate with lipopolysaccharide increased interleukin-1β production more than lipopolysaccharide alone. Butyrate with lipopolysaccharide increased caspase-1 activity more than lipopolysaccharide alone. As for the phosphorylation pathway, PD98059 (ERK1/2 inhibitor), SB203580 (p38 MAPK inhibitor), SP600125 (JNK1/2 inhibitor) decreased caspase-1 activity and interleukin-1β production to approximately 50% of the controls. Pertussis toxin (G protein-coupled signal transduction pathway inhibitor) also reduced interleukin-1β production to approximately 50%. Butyrate with lipopolysaccharide increased reactive oxygen species levels more than lipopolysaccharide alone. The addition of N-acetyl L-cysteine reduced reactive oxygen species levels to a level similar to that of lipopolysaccharide alone. Butyrate with lipopolysaccharide increased nitric oxide production more than lipopolysaccharide alone, and the addition of N-acetyl L-cysteine reduced the elevated amount of nitric oxide. In conclusions, butyrate enhances interleukin-1β production by activating caspase-1, via reactive oxygen species, the phosphorylation of MAPK, and G protein mediated pathways in lipopolysaccharide stimulated THP-1 cells. |
| Doi | 10.3164/jcbn.11-22 |
| Pmid | 22247602 |
| Wosid | WOS:000309324200010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000309324200010 |
| Is Public | Yes |
| Language Text | English |
| Keyword | singlet oxygen; titanium dioxide; 405 nm; electron spin resonance spectroscopy; photodynamic therapy |