Effects of gamma-amino butyric acid, progesterone and oestradiol on human spermatozoa acrosome reaction
Vigil, P; Orellana, R; Godoy, A; Barrientos, V; del Rio, MJ
| HERO ID | 1461454 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Effects of gamma-amino butyric acid, progesterone and oestradiol on human spermatozoa acrosome reaction |
| Authors | Vigil, P; Orellana, R; Godoy, A; Barrientos, V; del Rio, MJ |
| Page Numbers | 107-111 |
| Abstract | The acrosome reaction (AR) is influenced by the action of several molecules such as GABA. P(4). and ET Objective: to determine the interaction between GABA / E(2). and GABA / P(4) in the AR. Methods: Washed human spermatozoa capacitated for 2 h were incubated in the following conditions: BWW medium alone (control) or BWW plus: P(4) E(2) GABA, GABA+ P(4) or GABA+ E(2) The % of AR was determined by FITC-PSA.'ReSults: Spermatozoa incubated with -GABA or P(4) showed higher % of AR: additionally, separate and simultaneous P(4) and GABA incubations showed no significant difference in the % of AR. GABA plus E, incubation showed lower % of AR than GABA only incubated spermatozoa. Conclusions: The AR would be modulated by inductors such as GABA and P4, there is not synergistic interaction between these molecules on AR induction: besides, there are inhibitory hormones such as E,. whose effect prevails in a combined GABA/E, incubation. The localisation of these compounds would determine timely AR occunence. |
| Wosid | WOS:000278579300022 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000278579300022 Journal:PAPERS CONTRIBUTED TO THE 9TH INTERNATIONAL CONGRESS OF ANDROLOGY |
| Is Public | Yes |