Coupled oxidation-reduction of butanol-hexanal by resting Rhodococcus erythropolis NCIMB 13064 cells in liquid and gas phases
Marchand, P; Rosenfeld, E; Erable, B; Maugard, T; Lamare, S; Goubet, I
HERO ID
1462192
Reference Type
Journal Article
Year
2008
Language
English
| HERO ID | 1462192 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Coupled oxidation-reduction of butanol-hexanal by resting Rhodococcus erythropolis NCIMB 13064 cells in liquid and gas phases |
| Authors | Marchand, P; Rosenfeld, E; Erable, B; Maugard, T; Lamare, S; Goubet, I |
| Journal | Enzyme and Microbial Technology |
| Volume | 43 |
| Issue | 6 |
| Page Numbers | 423-430 |
| Abstract | Rhodococcus erythropolis is a promising Gram-positive bacterium capable of numerous bioconversions including those involving alcohol dehydrogenases (ADHs). In this work, we compared and optimized the redox biocatalytic performances of 1- butanol-grown R. erythropolis NCIMB 13064 cells in aqueous and in non-conventional gas phase using the 1-butanol-hexanal oxidation-reduction as model reaction. Oxidation of 1-butanol to butanal is tightly coupled to the reduction of hexanal to 1-hexanol at the level of a nicotinoprotein-ADH-like enzyme. Cell viability is dispensable for reaction. In aqueous batch conditions, fresh and lyophilized cells are efficient redox catalysts (oxidation-reduction rate = 765 mu mol min(-1) g cell dry mass(-1)) being also reactive towards benzyl alcohol, (S)-2- pentanol, and geraniol as reductants. However, butanol-hexanal oxidation-reduction is strongly limited by product accumulation and by hexanal toxicity that is a major factor influencing cell behavior and performance. Reaction rate is maximal at 40 degrees C-pH 7.0 in aqueous phase and at 60 degrees C-pH 7.0-9.0 in gas phase. Importantly, lyophilized cells also showed to be promising redox catalysts in the gas phase (at least 65 mu mol min(-1) g cell dry mass(-1)). The system is notably stable for several days at moderate thermodynamic activities of hexanal (0.06-0.12), 1- butanol (0.12) and water (0.7). (C) 2008 Elsevier Inc. All rights reserved. |
| Doi | 10.1016/j.enzmictec.2008.07.004 |
| Wosid | WOS:000260561800006 |
| Url | http://linkinghub.elsevier.com/retrieve/pii/S0141022908002135 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000260561800006 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Biocatalysis; Whole cells; Oxido-reduction; Rhodococcus erythropolis; Liquid phase; Solid/gas phase |