Disk to sphere transformation of erthrocyte induced by 1-anilino-8-nephthalene sulfonate (ANS): II. Quantitative study on adsorption of ANS by whole erythrocyte and by ghost membrane
Yoshida, S
| HERO ID | 1483107 |
|---|---|
| Year | 1976 |
| Title | Disk to sphere transformation of erthrocyte induced by 1-anilino-8-nephthalene sulfonate (ANS): II. Quantitative study on adsorption of ANS by whole erythrocyte and by ghost membrane |
| Authors | Yoshida, S |
| Volume | TOKYO |
| Page Numbers | 3039-3044 |
| Abstract | HEEP COPYRIGHT: BIOL ABS. Amounts of 1-anilino-8-naphthalene sulfonate (ANS) adsorbed by whole bovine erythrocyte and by its ghost membrane were studied at ANS concentration from 0.01-3 mM. For whole erythrocyte the amount is nearly proportional to the free ANS concentration with the ratio of 1.1ked-erythrocyte)/(mol/l-medium). The ghost membrane has 2 kinds of binding site for ANS. The Kd and the numbers of binding sites are calculated as follows: KI = 9.110-2 mol/kg-membrane for site J; KII = 1.0.8I can be attributed to protein and lipid, respectively, judging from membrane mass per mol of sites. The mechanism of disk to sphere transformation was discussed from these results. |
| Report Number | HEEP/77/09617 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: CHEM PHARM BULL ISSN: |
| Is Public | Yes |