Simultaneous exposure of english sole parophrys-vetulus to sediment-associated xenobiotics part 2. chronic exposure to an urban estuarine sediment with added tritiated benzo-a-pyrene and carbon-14 polychlorinated biphenyls
Stein, JE; Hom, T; Casillas, E; Friedman, A; Varanasi, U
HERO ID
1487116
Reference Type
Journal Article
Year
1987
Language
English
| HERO ID | 1487116 |
|---|---|
| In Press | No |
| Year | 1987 |
| Title | Simultaneous exposure of english sole parophrys-vetulus to sediment-associated xenobiotics part 2. chronic exposure to an urban estuarine sediment with added tritiated benzo-a-pyrene and carbon-14 polychlorinated biphenyls |
| Authors | Stein, JE; Hom, T; Casillas, E; Friedman, A; Varanasi, U |
| Journal | Marine Environmental Research |
| Volume | 22 |
| Issue | 2 |
| Page Numbers | 123-150 |
| Abstract | 1. The accumulation of aromatic hydrocarbons (AHs) and polychlorinated biphenyls (PCBs) kv English sole (Parophrys vetulus) exposed for up to 108 days to an urban (test) sediment was compared to that for English sole exposed to sediment from a reference area. The concentrations of identified AHs and PCBs in the test sediment were 32 μg/g dry weight and 2·2 μg/g dry weight, respectively. English sole exposed to test and reference sediments for 108 days had biliary concentrations of aromatic compounds fluorescing at BaP wavelengths of 0·6 μg/g, wet weight and hepatic concentrations of PCBs of 1·4 ± 0·6 μg/g wet weight which were ten and eight times, respectively, as great as those in reference sole. These results show that accumulation of AHs and PCBs from sediment by English sole is a significant route of uptake in contaminated environments. 2. 3H-benzo(a)pyrene (BaP) and 14C-PCBs were added to the test sediment to compare the uptake and metabolism of BaP to that of PCBs by sole. Steady-state concentrations of 3H-BaP and 14C-PCBs in tissues and fluids were reached by 4 days and 14 days, respectively. At steady state, the level of covalent binding of 3H-BaP to hepatic proteins in test sole was six times greater than that of 14C-PCBs, and there was significantly greater relative formation of BaP-glutathione (GSH) conjugates than PCB-GSH conjugates. Thus, the amount of BaP metabolized to reactive metabolites was greater than that for PCBs, even though the concentration of 14C-PCBs in liver of test sole was five times as great as that of 3H-BaP. 3. Trace amounts of 3H-BaP were also added to the reference sediment to determine how simultaneous exposure of English sole to those contaminants already present in the test sediment affected the metabolism and disposition of BaP. The 3H-BaP concentration ratios for liver to sediment and bile to sediment, as well as proportions of biliary BaP-GSH conjugates in test sole, were significantly greater than in reference sole. Thus, the formation and accumulation of potentially toxic metabolites of a carcinogenic AH (BaP) in sole liver were increased by simultaneous exposure to contaminants present in an urban sediment. |
| Doi | 10.1016/0141-1136(87)90032-8 |
| Wosid | WOS:A1987H792900003 |
| Is Certified Translation | No |
| Dupe Override | 1487116 |
| Is Public | Yes |
| Language Text | English |
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