Biochemical and microbial analysis of ovine rumen fluid incubated with 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX)
Perumbakkam, S; Craig, AM
HERO ID
1506933
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1506933 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Biochemical and microbial analysis of ovine rumen fluid incubated with 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX) |
| Authors | Perumbakkam, S; Craig, AM |
| Journal | Current Microbiology |
| Volume | 65 |
| Issue | 2 |
| Page Numbers | 195-201 |
| Abstract | In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M-O + HCOO) and methylenedinitramine metabolites. A total of 1106 16S rRNA-V3 clones were sequenced, and most sequences associated with either the phyla Bacteroidetes or Firmicutes. A LibCompare analysis for the RDX treatment showed an enrichment (P < 0.01) of the genus Prevotella. From these results, it can be concluded that the rumen is capable of detoxifying RDX, and the members of the genus Prevotella are linked to this detoxification. |
| Doi | 10.1007/s00284-012-0144-1 |
| Pmid | 22614100 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |