Limitations of the lignin peroxidase system of the white-rot fungus, Phanerochaete chrysosporium

Kay-Shoemake, JL; Watwood, ME

HERO ID

1579677

Reference Type

Journal Article

Year

1996

Language

eng

HERO ID 1579677
In Press No
Year 1996
Title Limitations of the lignin peroxidase system of the white-rot fungus, Phanerochaete chrysosporium
Authors Kay-Shoemake, JL; Watwood, ME
Journal Applied Microbiology and Biotechnology
Volume 46
Issue 4
Page Numbers 438-442
Abstract BIOSIS COPYRIGHT: BIOL ABS. The lignin peroxidase enzyme system of the white-rot fungus, Phanerochaete chrysosporium, was assayed for its capacity to degrade two recalcitrant aliphatic ether compounds, high-molecular-mass polyethylene glycol (PEG 20000) and methyl tert-butyl ether. Ligninolytic cultures of Phanerochaete chrysosporium were spiked with each ether compound and incubated in reaction vessels. Separate incubations were conducted in which the ether compounds were present as sole carbon source. Other parameters, such as varying the methyl tert-butyl ether concentration and veratryl alcohol additions were tested. No significant degradation of either compound was observed under any of the conditions tested. Implications of these results are discussed with respect to the oxidative limitations of the lignin peroxidase enzyme system and structural features of substrate molecules that may be requisite for oxidation by this system.
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Language Text eng
Keyword <?xml version="1.0" encoding="UTF-8"?><kw>Biochemical Studies-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biophysics-Molecular Properties and Macromolecules</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Metabolism-General Metabolism</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Food and Industrial Microbiology-Biodegradation and Biodeterioration</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Plant Physiology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Plant Physiology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Basidiomycetes</kw>