Preparation of synthetic human islet amyloid polypeptide (IAPP) in a stable conformation to enable study of conversion to amyloid-like fibrils
Higham, CE; Jaikaran, ET; Fraser, PE; Gross, M; Clark, A
| HERO ID | 1614380 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Preparation of synthetic human islet amyloid polypeptide (IAPP) in a stable conformation to enable study of conversion to amyloid-like fibrils |
| Authors | Higham, CE; Jaikaran, ET; Fraser, PE; Gross, M; Clark, A |
| Journal | FEBS Letters |
| Volume | 470 |
| Issue | 1 |
| Page Numbers | 55-60 |
| Abstract | Human synthetic islet amyloid polypeptide (hIAPP) is rapidly converted to beta-sheet conformation and fibrils in aqueous media. Optimal solubility conditions for hIAPP were determined by circular dichroism spectroscopy and transmission electron microscopy. hIAPP in trifluoroethanol or hexafluoro-2-isopropanol (HFIP) diluted in water or phosphate buffer (PB) exhibited random structure which was converted to beta-sheet and fibrils with time. hIAPP, solubilised in HFIP, filtered and lyophilised remained in stable random structure for up to 7 days in water; in PB, insoluble aggregates precipitated from which protofilaments and fibrils formed with time. This suggests that amorphous aggregates of hIAPP could initiate islet amyloidosis in vivo. |
| Doi | 10.1016/s0014-5793(00)01287-4 |
| Pmid | 10722845 |
| Wosid | WOS:000086001800012 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000086001800012 |
| Is Public | Yes |
| Language Text | English |
| Keyword | islet amyloid polypeptide; fibril; beta-sheet; amyloid |