In vitro and in vivo comparisons of fish-specific CYP1A induction relative potency factors for selected polycyclic aromatic hydrocarbons
Billiard, SM; Bols, NC; Hodson, PV
HERO ID
1617816
Reference Type
Journal Article
Year
2004
Language
English
PMID
| HERO ID | 1617816 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | In vitro and in vivo comparisons of fish-specific CYP1A induction relative potency factors for selected polycyclic aromatic hydrocarbons |
| Authors | Billiard, SM; Bols, NC; Hodson, PV |
| Journal | Ecotoxicology and Environmental Safety |
| Volume | 59 |
| Issue | 3 |
| Page Numbers | 292-299 |
| Abstract | Induction of cytochrome P450 (CYP1A), as measured by liver ethoxyresorufin-O-deethylase (EROD) activity in juvenile rainbow trout (Oncorhynchus mykiss), was used to derive relative potency factors (RPFs) for several polycyclic aromatic hydrocarbons (PAHs), chosen for their induction potency in a rainbow trout liver cell line (RTL-W1). Potency for causing induction was estimated as the median effective concentration (EC50) from exposure-response curves. With the exception of phenanthrene, all PAHs tested induced EROD activity in juvenile trout, ranked as: benzo[k]fluoranthene>benzo[b]fluoranthene>benzo[b]fluorene>beta-napthoflavone>retene (7-isopropyl-1-methylphenanthrene). When induction potency was expressed relative to benzo[k]fluoranthene, RPFs ranged from 0.02 to 1, and the rank order in vivo was identical to the rank order with RTL-W1-derived values. The additivity of PAHs in mixtures in RTL-W1 cells was compared to whole-fish results from a previous study. EROD induction showed additive interactions for PAHs with exposure-response curves of similar slopes. This study demonstrates that assays of CYP1A induction using rainbow trout liver cells in culture would be a convenient substitute for assays with whole fish as part of testing programs for risk assessment of PAHs. |
| Doi | 10.1016/j.ecoenv.2004.06.009 |
| Pmid | 15388268 |
| Wosid | WOS:000224318700002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-4544289442&doi=10.1016%2fj.ecoenv.2004.06.009&partnerID=40&md5=b2ac9a77ac2ba2fac51f818b0648c399 |
| Is Public | Yes |
| Language Text | English |
| Keyword | TEFs; RPFs; TCDD; polycyclic aromatic hydrocarbons; RTL-W1; CYP1A; juvenile trout |