Purification and crystallization of a catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus BR219
Sugimoto, K; Senda, T; Masai, E; Kimbara, K; Fukuda, M; Mitsui, Y
| HERO ID | 1630550 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Purification and crystallization of a catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus BR219 |
| Authors | Sugimoto, K; Senda, T; Masai, E; Kimbara, K; Fukuda, M; Mitsui, Y |
| Journal | Protein and Peptide Letters |
| Volume | 6 |
| Issue | 6 (Dec 1999) |
| Page Numbers | 403-406 |
| Abstract | The thermostable catechol 2,3-dioxygenase from Bacillus stearothermophilus BR219 has been crystallized by vapor-diffusion method. The crystals were grown from a solution containing isopropanol, HEPES and magnesium chloride. The crystals belonged to the monoclinic system with a space group P2 sub(1) and unit cell dimensions of a=69.4Angstrom, b=61.5Angstrom, c=153.1Angstrom and beta =92.6 degree . |
| Wosid | WOS:000084636000009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000084636000009 |
| Is Public | Yes |
| Keyword | Catechol 2,3-dioxygenase; Bacillus stearothermophilus; PheB protein; Solutions; Crystallization |