Characterization of a Methanosarcina strain isolated from goat feces, and that grows on H sub(2)-CO sub(2) only after adaptation
Mukhopadhyay, B; Purwantini, E; de Macario, EC; Daniels, L
| HERO ID | 1630727 |
|---|---|
| In Press | No |
| Year | 1991 |
| Title | Characterization of a Methanosarcina strain isolated from goat feces, and that grows on H sub(2)-CO sub(2) only after adaptation |
| Authors | Mukhopadhyay, B; Purwantini, E; de Macario, EC; Daniels, L |
| Journal | Current Microbiology |
| Volume | 23 |
| Issue | 3 (1991) |
| Page Numbers | 165-173 |
| Abstract | A Methanosarcina species, designated strain ChGul, was isolated from goat feces; this is the first fully described pure culture of Methanosarcina obtained from feces. Antigenic fingerprinting suggests that isolate ChGul is a new immunotype. The mol% G + C content of DNA was 42.2%. Strain ChGul grew on methanol, methylamines, and acetate in a minimal salts medium. It grew on H sub(2)-CO sub(2) only after adaptation. Growth occurred as a milky-white suspension and contained cells mostly in doublets and quadruplets of irregular cocci; many cells contained phase bright spots typical of gas vacuoles. The isolate did not grow on formate, or CO sub(2) plus isopropanol, ethanol, or acetone as substrates and did not produce methane from formate. The optimum growth temperature was 35-37 degree C, and optimum pH was 6.2-6.8. ChGul is unusually sensitive to sulfide and has low tolerance for NaCl. Optimal levels of total sulfide and NaCl for growth were 0.5 mM and 20-40 mM, respectively. |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1991GD19400008 |
| Is Public | Yes |
| Keyword | feces; methanogenesis |
| Is Qa | No |
| Relationship(s) |
|