Ethanol causes the redistribution of L1 cell adhesion molecule in lipid rafts
Tang, N; Farah, B; He, M; Fox, S; Malouf, A; Littner, Y; Bearer, CF
HERO ID
1641978
Reference Type
Journal Article
Year
2011
Language
English
PMID
| HERO ID | 1641978 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Ethanol causes the redistribution of L1 cell adhesion molecule in lipid rafts |
| Authors | Tang, N; Farah, B; He, M; Fox, S; Malouf, A; Littner, Y; Bearer, CF |
| Journal | Journal of Neurochemistry |
| Volume | 119 |
| Issue | 4 |
| Page Numbers | 859-867 |
| Abstract | Fetal alcohol spectrum disorder is estimated to affect 1% of live births. The similarities between children with fetal alcohol syndrome and those with mutations in the gene encoding L1 cell adhesion molecule (L1) implicates L1 as a target of ethanol developmental neurotoxicity. Ethanol specifically inhibits the neurite outgrowth promoting function of L1 at pharmacologic concentrations. Emerging evidence shows that localized disruption of the lipid rafts reduces L1-mediated neurite outgrowth. We hypothesize that ethanol impairment of the association of L1 with lipid rafts is a mechanism underlying ethanol's inhibition of L1-mediated neurite outgrowth. In this study, we examine the effects of ethanol on the association of L1 and lipid rafts. We show that, in vitro, L1 but not N-cadherin shifts into lipid rafts following treatment with 25 mM ethanol. The ethanol concentrations causing this effect are similar to those inhibiting L1-mediated neurite outgrowth. Increasing chain length of the alcohol demonstrates the same cutoff as that previously shown for inhibition of L1-L1 binding. In addition, in cerebellar granule neurons in which lipid rafts are disrupted with methyl-beta-cyclodextrin, the rate of L1-mediated neurite outgrowth on L1-Fc is reduced to background rate and that this background rate is not ethanol sensitive. These data indicate that ethanol may inhibit L1-mediated neurite outgrowth by retarding L1 trafficking through a lipid raft compartment. |
| Doi | 10.1111/j.1471-4159.2011.07467.x |
| Pmid | 21884525 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |