COBALAMIN-ENHANCED ANAEROBIC BIOTRANSFORMATION OF CARBON-TETRACHLORIDE
Hashsham, SA; Scholze, R; Freedman, DL
HERO ID
1658559
Reference Type
Journal Article
Year
1995
Language
English
PMID
| HERO ID | 1658559 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | COBALAMIN-ENHANCED ANAEROBIC BIOTRANSFORMATION OF CARBON-TETRACHLORIDE |
| Authors | Hashsham, SA; Scholze, R; Freedman, DL |
| Journal | Environmental Science & Technology |
| Volume | 29 |
| Issue | 11 |
| Page Numbers | 2856-2863 |
| Abstract | Biotransformation of carbon tetrachloride (CT) was examined with an anaerobic enrichment culture grown on dichloromethane as the sole organic carbon and energy source. The principal products from [C-14]-CT included chloroform (17%), carbon disulfide (21%), and CO2 (21%). When cyanocobalamin was added along with CT, the percentage converted to CO2 increased almost 3-fold (59%), while CS2 decreased somewhat (11%), and virtually none of the CT (<1%) was reduced to chloroform. Carbon monoxide was a major transformation product (12-27%) in autoclaved cultures and in live cultures that received high levels of CT (up to 52 mg/L). Adding cyanocobalamin also increased the rate of CT transformation in live cultures by at least 10-fold, but had a minor effect on the rate of CT use in autoclaved cultures. Accelerated rates of transformation by live cultures were sustained for as long as 200 days, with hydrogen serving as the electron donor. Cyanocobalamin, hydroxocobalamin, and methylcobalamin were equally effective, while a 3-week lag period was required before adenosylcobalamin started to enhance CT transformation. Because of their high cost, the feasibility of using cobalamins will depend on the amount required. We observed significant enhancement in CT transformation at concentrations up to 340 mu M, with cobalamin levels as low as 10 mu M. |
| Doi | 10.1021/es00011a023 |
| Pmid | 22206535 |
| Wosid | WOS:A1995TC85600041 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1995TC85600041 |
| Is Public | Yes |
| Language Text | English |