Penetration Enhancing Effect of Polysorbate 20 and 80 on the In Vitro Percutaneous Absorption of L-Ascorbic Acid
Akhtar, N; Rehman, MU; Khan, HMS; Rasool, F; Saeed, T; Murtaza, G
| HERO ID | 1765419 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Penetration Enhancing Effect of Polysorbate 20 and 80 on the In Vitro Percutaneous Absorption of L-Ascorbic Acid |
| Authors | Akhtar, N; Rehman, MU; Khan, HMS; Rasool, F; Saeed, T; Murtaza, G |
| Journal | Tropical Journal of Pharmaceutical Research |
| Volume | 10 |
| Issue | 3 |
| Page Numbers | 281-288 |
| Abstract | Purpose: To investigate the penetration enhancing effect of two polysorbates - polyoxyethylene 20 (POE-20) and polyoxyethylene 80 (POE-80) - on the in vitro percutaneous absorption of ascorbic acid (AA). Methods: For the permeation experiments, Franz diffusion cell covered with aluminum foil providing an effective diffusion area of 1.76 cm(2) and hairless rabbit skin were used. A range of concentrations (1 - 5 %) of POE-20 and POE-80 was added to the ascorbic acid to determine their optimum enhancement concentration. Results: The cumulative amount of AA that diffused across the skin increased with increase in the concentration of the permeation enhancers. Without the enhancer, AA flux was 0.626 mu g/cm(2)/h while mean permeability coefficient (K(p)) was 2.09 x 10(-6) cm/h. AA flux was 3.17 and 2.44 mu g/cm(2)/h for POE-20 and POE-80, respectively, while mean permeability coefficient was 10.6 x 10 (-6) and 8.14 x 10(-6) K(p), cm/h. Maximum flux (3.16 mu g/cm(2)/h) at POE-20 concentration of 5 % was obtained, with an enhancement ratio (ER) of 5.07 in relation to control (i.e., AA without enhancer). For POE-80 (5 %), maximum flux was 2.44 mu g/cm(2)/h with an ER value of 3.89, compared to control. Conclusion: This study demonstrates that POE-20 and POE-80 exerted a penetration enhancing effect on the percutaneous absorption of L-ascorbic acid (AA). |
| Doi | 10.4314/tjpr.v10i3.1 |
| Wosid | WOS:000298173600007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000298173600007 |
| Is Public | Yes |
| Keyword | L-Ascorbic acid; Skin permeation; Polyoxyethylene (Polysorbate); Flux; Penetration enhancers |