Analysis of paraphenylenediamine
Bellimam, MA; Stambouli, A; El Karni, N; Bouayoun, T; El Bouri, A
| HERO ID | 1808061 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Analysis of paraphenylenediamine |
| Authors | Bellimam, MA; Stambouli, A; El Karni, N; Bouayoun, T; El Bouri, A |
| Journal | Acta Clinica Belgica. Supplementum |
| Volume | 61 Suppl 1 |
| Issue | 1 |
| Page Numbers | 41-47 |
| Abstract | The toxic Paraphenylenediamine is characterized by infrared spectrophotometry and nuclear magnetic resonance spectroscopy, whereas, high performance liquid chromatography with a refractive index detector was used to determine its purity in the suspect samples using external standardisation. An analytical method for determination of lower traces of paraphenylenediamine in post-mortem biological fluids was developed. This procedure involves deproteneization or hydrolysis followed by liquid-liquid extraction and derivatization with trifluoroacetic anhydride. 1 microL of the extract was then analysed by gas chromatography/iontrap mass spectrometry. Benzidine used as the internal standard for quantification and the extraction recovery test was evaluated to 85%. This method was validated in cases with paraphenylenediamine poisoning. |
| Doi | 10.1179/acb.2006.068 |
| Pmid | 16700150 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |