Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
Miyano, Y; Tsukuda, S; Sakimoto, I; Takeuchi, R; Shimura, S; Takahashi, N; Kusayanagi, T; Takakusagi, Y; Okado, M; Matsumoto, Y; Takakusagi, K; Takeuchi, T; Kamisuki, S; Nakazaki, A; Ohta, K; Miura, M; Kuramochi, K; Mizushina, Y; Kobayashi, S; Sugawara, F; Sakaguchi, K
HERO ID
1937237
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1937237 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen |
| Authors | Miyano, Y; Tsukuda, S; Sakimoto, I; Takeuchi, R; Shimura, S; Takahashi, N; Kusayanagi, T; Takakusagi, Y; Okado, M; Matsumoto, Y; Takakusagi, K; Takeuchi, T; Kamisuki, S; Nakazaki, A; Ohta, K; Miura, M; Kuramochi, K; Mizushina, Y; Kobayashi, S; Sugawara, F; Sakaguchi, K |
| Journal | Bioorganic & Medicinal Chemistry |
| Volume | 20 |
| Issue | 13 |
| Page Numbers | 3985-3990 |
| Abstract | Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells. |
| Doi | 10.1016/j.bmc.2012.05.016 |
| Pmid | 22658539 |
| Wosid | WOS:000305279700004 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Perfluorooctane sulfonate; Solid-phase synthesis; Phage display; CD14; RELIC |