Gene-locus mutation assays in diploid human lymphoblast lines
Thilly, WG; Deluca, JG; Furth, EE; Hoppe, H; Kaden, DA; Krolewski, JJ; Liber, HL; Skopek, TR; Slapikoff, SA; Tizard, RJ; Penman, BW
HERO ID
2135193
Reference Type
Book/Book Chapter
Year
1980
Language
English
| HERO ID | 2135193 |
|---|---|
| Year | 1980 |
| Title | Gene-locus mutation assays in diploid human lymphoblast lines |
| Book Title | Chemical mutagens: Principles and methods for their detection |
| Authors | Thilly, WG; Deluca, JG; Furth, EE; Hoppe, H; Kaden, DA; Krolewski, JJ; Liber, HL; Skopek, TR; Slapikoff, SA; Tizard, RJ; Penman, BW |
| Editor | de Serres, FJ; Hollaender, A |
| Publisher Text | Plenum Press |
| City | New York, NY |
| Volume | 6 |
| Page Numbers | 331-364 |
| Abstract | Our primary reasons for using diploid human lymphoblast lines in studies of mutagenesis are that they can be grown in free suspension; they appear to be genetically stable for chromosome number, as well as immortal; and they are readily derived from humans of different genetic backgrounds and possibly different sensitivities to mutagenic agents. Of these properties, the most important in assessing the mutagenicity of a large number of suspect chemicals is the lymphoblasts' ability to grow in suspension culture. This characteristic offers the potential for preprogrammed, automatic handling, which probably could not be achieved with anchorage-dependent cells. In this chapter, we will introduce the basics of lymphoblast husbandry and some protocols (tricks) that we have used to facilitate their use in studying genetic change. |
| Doi | 10.1007/978-1-4613-3072-1_12 |
| Is Certified Translation | No |
| Dupe Override | No |
| Isbn | 9781461330745 |
| Comments | Journal: CHEM MUTAGENS 6 ISSN: |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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