Effect of isomerically pure chlorobiphenyls on structure of kidney tubules in rat

Hansell, MM; Ecobichon, DJ

HERO ID

2165125

Reference Type

Journal Article

Subtype

Abstract

Year

1974

Language

English

HERO ID 2165125
Material Type Abstract
In Press No
Year 1974
Title Effect of isomerically pure chlorobiphenyls on structure of kidney tubules in rat
Authors Hansell, MM; Ecobichon, DJ
Journal Toxicology and Applied Pharmacology
Volume 29
Issue 1
Page Numbers 102-103
Abstract Commercial polychlorinated biphenyls are mixtures of various compounds with only the total chlorination determined. Studies of the effects of isomerically-pure chlorobiphenyls on liver carried out in these laboratories showed that the effect of the compound was related to the position and degree of chlorination. In a parallel study, an attempt was made to determine if there was a similar relationship in kidney. The agents used were Aroclors 1254 and 1260, biphenyl and a series of isomerically-pure mono-, di-, tetra-, hexa-, and octochloro-biphenyls. Groups of 50-60 g male Woodlyn Wistar rats were injected (ip) with agent dissolved in peanut oil at a dosage of 50 mg/kg/day for 3 days, and killed with chloroform 4 days after the final injection. Samples of kidney were immediately fixed by immersion in 1% OsO4 for electron microscopy. In a second experiment, groups of rats were injected with 3 compounds ( 4-mono-, 4,4' -di- and 2,5,2',5'-tetrachlorobiphenyl) at a rate of 100 mg/kg/day for 7 days and killed 1 day later. The glomerulus appeared to be unaffected by treatment, but alterations could be found in renal tubule cells. These included increases in the number of vacuoles, lipid droplets, microbodies, lysosomes and smooth endoplasmic reticulum. Some tubule cells contained mitochondria having altered morphology and disruption of the ordered appearance of the basal in foldings of the plasma membrane. In both experiments, biphenyl and mono- and dichlorobiphenyls caused more marked changes than did the more highly chlorinated compounds.
Wosid WOS:A1974T653800076
Is Certified Translation No
Dupe Override No
Conference Location Washington, DC
Conference Name Thirteenth Annual Meeting of the Society of Toxicology
Conference Date March 10-14, 1974
Is Public Yes
Language Text English
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