Butyric acid-induced rat jugular blood cytosolic oxidative stress is associated with SIRT1 decrease
Cueno, ME; Imai, K; Tamura, M; Ochiai, K
HERO ID
2234205
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2234205 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Butyric acid-induced rat jugular blood cytosolic oxidative stress is associated with SIRT1 decrease |
| Authors | Cueno, ME; Imai, K; Tamura, M; Ochiai, K |
| Journal | Cell Stress and Chaperones |
| Volume | 19 |
| Issue | 2 |
| Page Numbers | 295-298 |
| Abstract | Butyric acid (BA) induces jugular blood mitochondrial oxidative stress, whereas heme-induced oxidative stress was previously reported to inhibit SIRT1 in vitro. This would imply that BA-induced oxidative stress may similarly affect SIRT1. Here, we elucidated the BA effects on jugular blood cytosolic oxidative stress and SIRT1. Jugular blood cytosol was collected 0, 60, and 180 min after BA injection into rat gingival tissues and used throughout the study. Blood cytosolic oxidative stress induction, heme accumulation, NADPH oxidase (NOX) activation, nicotinamide adenine dinucleotide (NAD(+)) and NADP pool levels, NAD kinase (NADK), and SIRT1 amounts were determined. We found that BA retention in the gingival tissue induces blood cytosolic oxidative stress and heme accumulation which we correlated to both NOX activation and NADP pool increase. Moreover, we showed that BA-related NADP pool build-up is associated with NADK increase which we suspect decreased NAD(+) levels and consequentially lowered SIRT1 amounts in the rat blood cytosol. |
| Doi | 10.1007/s12192-013-0462-7 |
| Pmid | 24052229 |
| Wosid | WOS:000331969600014 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Butyric acid; NADPH oxidase; Oxidative stress; Rat blood cytosol; Sirtuin-1 |