Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells
Liu, Q; Liu, J; Roschmann, KIL; van Egmond, D; Golebski, K; Fokkens, WJ; Wang, D; van Drunen, CM
HERO ID
2280270
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 2280270 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells |
| Authors | Liu, Q; Liu, J; Roschmann, KIL; van Egmond, D; Golebski, K; Fokkens, WJ; Wang, D; van Drunen, CM |
| Journal | Journal of Inflammation |
| Volume | 10 |
| Issue | 1 |
| Page Numbers | 15 |
| Abstract | HDAC inhibitors have been proposed as anticancer agents. However, their roles in innate genes expression remain not well known. Cathelicidin LL-37 is one of the few human bactericidal peptides, but the regulation of histone acetylation on LL-37 expression in airway epithelium remains largely unknown. Therefore, we investigated the effects of two non-selective HDACi, trichostatin A (TSA) and sodium butyrate (SB), on the expression of the cathelicidin LL-37 in human airway epithelial cells. LL37 in human NCI-H292 airway epithelial cells and the primary cultures of normal nasal epithelial cells(PNEC) in response to HDAC inhibitors with or without poly (I:C) stimulation was assessed using real-time PCR and western blot. In parallel, IL-6 expression was evaluated by ELISA. Our results showed that HDAC inhibitors up-regulated LL-37 gene expression independent of poly (I:C) stimulation in PNEC as well as in NCI-H292 cells. HDAC inhibitors increased LL37 protein expression in NCI-H292 cells but not in PNEC. In addition, HDAC inhibitors significantly inhibited poly (I:C)-induced IL-6 production in both of the epithelial cells. In conclusion, HDAC inhibitors directly up-regulated LL-37 gene expression in human airway epithelial cells. |
| Doi | 10.1186/1476-9255-10-15 |
| Pmid | 23577829 |
| Wosid | WOS:000318607600001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Epigenetics; Innate immunity; Histone; Deacetylation; Toll-like receptor; Cathelicidins |