Application of radiorespirometry as a fast screening method for toxic substances: 1. Effect of methylmercury chloride
Lee, SD; Danner, RM; Mcmillan, L; Iltis, R
HERO ID
2306192
Reference Type
Journal Article
Subtype
Abstract
Year
1974
Language
English
| HERO ID | 2306192 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 1974 |
| Title | Application of radiorespirometry as a fast screening method for toxic substances: 1. Effect of methylmercury chloride |
| Authors | Lee, SD; Danner, RM; Mcmillan, L; Iltis, R |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 29 |
| Issue | 1 |
| Page Numbers | 147-148 |
| Abstract | The primary objective of this investigation is to develop new, or apply existing techniques that are capable of detecting early biochemical changes before the appearance of overt toxic signs in the process of toxicologic assessment of low concentrations of environmental pollutants. Various reports have been published on biologic effects of pollutants; yet, there is a paucity of information concerning the effects of pollutants on biochemical interactions at low, relevant concentrations of environmental pollutants using in vivo nonterminal experiments. Rats exposed to S02 showed decreased metabolic activity, as determined by suppressed CO2 output following injection of [U-14C]glucose; the degree of suppression was related to SO2 concentration between 27.7 and 200 ppm. On the other hand, 14CO2 release from rats following [1-14C]palmitate injection was enhanced. These phenomena indicated an effect similar to "Pasteur effect." Radiorespirometric investigation on metabolic effect of CH3HgCl provided an extremely sensitive measure. Intragastric administration of CH3HgCl (0.05 and 0.10 mg/kg) caused suppression in 14C02 output following an iv injection of [1-14C]glucose. This effect was cumulative when treatment was repeated 1 week later. To date the alterations reported herein are the earliest effects observed after exposure to low concentrations of CH3HgCl. These metabolic alterations were demonstrable in non-terminal in vivo experiments. Furthermore, a mathematical model has been developed from these results which enables one to predict the effect over a longer period of time by examining the 14CO2 excretion pattern over a 30- to 60-min period (initial slope). In addition, one can predict dose-response over a wide range of toxicant concentrations by simulation on analog computer and interpolation following a small number of strategic experiments. |
| Wosid | WOS:A1974T653800189 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Washington, D.C. |
| Conference Name | Thirteenth Annual Meeting of the Society of Toxicology |
| Conference Date | March 10–14, 1974 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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