Nitric oxide and nitroxides can act as efficient scavengers of protein-derived free radicals
Lam, MA; Pattison, DI; Bottle, SE; Keddie, DJ; Davies, MJ
HERO ID
2316001
Reference Type
Journal Article
Subtype
Abstract
Year
2008
Language
English
| HERO ID | 2316001 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2008 |
| Title | Nitric oxide and nitroxides can act as efficient scavengers of protein-derived free radicals |
| Authors | Lam, MA; Pattison, DI; Bottle, SE; Keddie, DJ; Davies, MJ |
| Journal | Free Radical Biology and Medicine |
| Volume | 45 |
| Issue | Suppl. |
| Page Numbers | S113-S113 |
| Abstract | Nitric oxide (•NO) may act as either a pro- or anti-oxidant in biological systems. Although •NO and nitroxide radicals react slowly with most molecules, they react at near diffusion-controlled rates with other radicals and may therefore be efficient protective agents. This study assessed the ability of •NO and nitroxides to intercept specific protein-derived radicals, and compared the efficacy of these species. Three protein radical systems were investigated: BSA-derived radicals generated via radical transfer from H2O2-activated horseradish peroxidase; radicals formed on myoglobin via reaction with H2O2; and carbon-centred radicals formed from amino-acid hydroperoxides on exposure to Fe2+- EDTA. in each case radicals were generated in the absence or presence of •NO or nitroxides of different size and charge. Concentration-dependent loss of the protein radicals was detected by EPR with both •NO and nitroxides, and time-dependent consumption of •NO using an •NO electrode. The protein oxidation product dityrosine was significantly reduced by •NO and nitroxides, and DOPA levels were reduced by nitroxides but not •NO. Overall, these studies demonstrate that •NO and nitroxides are efficient scavengers of protein radicals, and hence are potential protective agents against protein oxidation reactions and the resulting damage. These reactions show little dependence on nitroxide structure or charge. |
| Wosid | WOS:000260867900320 |
| Url | http://www.sciencedirect.com/science/article/pii/S089158490800628X |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Indianapolis, IN |
| Conference Name | Society for Free Radical Biology and Medicine 15th Annual Meeting |
| Conference Date | November 19-23, 2008 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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