Low Cytotoxicity of Inorganic Nanotubes and Fullerene-Like Nanostructures in Human Bronchial Epithelial Cells: Relation to Inflammatory Gene Induction and Antioxidant Response
Pardo, M; Shuster-Meiseles, T; Levin-Zaidman, S; Rudich, A; Rudich, Y
HERO ID
2340597
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2340597 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Low Cytotoxicity of Inorganic Nanotubes and Fullerene-Like Nanostructures in Human Bronchial Epithelial Cells: Relation to Inflammatory Gene Induction and Antioxidant Response |
| Authors | Pardo, M; Shuster-Meiseles, T; Levin-Zaidman, S; Rudich, A; Rudich, Y |
| Journal | Environmental Science & Technology |
| Volume | 48 |
| Issue | 6 |
| Page Numbers | 3457-3466 |
| Abstract | The cytotoxicity of tungsten disulfide nano tubes (INT-WS2) and inorganic fullerene-like molybdenum disulfide (IF-MoS2) nanoparticles (NPs) used in industrial and medical applications was evaluated in comparison to standard environmental particulate matter. The IF-MoS2 and INT-WS2 reside in vesicles/inclusion bodies, suggestive of endocytic vesicles. In cells representing the respiratory, immune and metabolic systems, both IF-MoS2 and INT-WS2 NPs remained nontoxic compared to equivalent concentrations (up to 100 μg/mL in the medium) of silica dioxide (SiO2), diesel engine-derived and carbon black NPs, which induced cell death. Associating with this biocompatibility of IF-MoS2\INT-WS2, we demonstrate in nontransformed human bronchial cells (NL-20) relative low induction of the pro-inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α. Moreover, IF-MoS2 and INT-WS2 activated antioxidant response as measured by the antioxidant response element (ARE) using a luciferase reporter, and induced Nrf2-mediated Phase II detoxification genes. Collectively, our findings suggest that the lower cytotoxicity of IF-MoS2 and INT-WS2 NPs does not reflect general biological inertness. Rather, compared to other NP's, it likely results from decreased pro-inflammatory activation, but a comparable significant capacity to induce protective antioxidant/detoxification defense mechanisms. |
| Doi | 10.1021/es500065z |
| Pmid | 24533583 |
| Wosid | WOS:000333776000045 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Antioxidants/analysis/metabolism; Cell Line; Cell Survival/drug effects; Cytokines/analysis/genetics/metabolism; Disulfides/toxicity; Epithelial Cells/drug effects; Fullerenes/toxicity; Gene Expression/drug effects; Molybdenum/toxicity; Nanotubes/toxicity; Particulate Matter/metabolism; Silicon Dioxide/toxicity; Soot/toxicity; Tungsten/toxicity |