Primary tumor genotype is an important determinant in identification of lung cancer propagating cells
Curtis, SJ; Sinkevicius, KW; Li, D; Lau, AN; Roach, RR; Zamponi, R; Woolfenden, AE; Kirsch, DG; Wong, KK; Kim, CF
HERO ID
2473956
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 2473956 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Primary tumor genotype is an important determinant in identification of lung cancer propagating cells |
| Authors | Curtis, SJ; Sinkevicius, KW; Li, D; Lau, AN; Roach, RR; Zamponi, R; Woolfenden, AE; Kirsch, DG; Wong, KK; Kim, CF |
| Journal | Cell Stem Cell |
| Volume | 7 |
| Issue | 1 |
| Page Numbers | 127-133 |
| Abstract | Successful cancer therapy requires the elimination or incapacitation of all tumor cells capable of regenerating a tumor. Therapeutic advances therefore necessitate the characterization of the cells that are able to propagate a tumor in vivo. We show an important link between tumor genotype and isolation of tumor-propagating cells (TPCs). Three mouse models of the most common form of human lung cancer each had TPCs with a unique cell-surface phenotype. The cell-surface marker Sca1 did not enrich for TPCs in tumors initiated with oncogenic Kras, and only Sca1-negative cells propagated EGFR mutant tumors. In contrast, Sca1-positive cells were enriched for tumor-propagating activity in Kras tumors with p53 deficiency. Primary tumors that differ in genotype at just one locus can therefore have tumor-propagating cell populations with distinct markers. Our studies show that the genotype of tumor samples must be considered in studies to identify, characterize, and target tumor-propagating cells. |
| Doi | 10.1016/j.stem.2010.05.021 |
| Pmid | 20621056 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |