Demonstration of an olfactory bulb-brain translocation pathway for ZnO nanoparticles in rodent cells in vitro and in vivo
Kao, YY; Cheng, TJ; Yang, DM; Wang, CT; Chiung, YM; Liu, PS
HERO ID
2564306
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 2564306 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Demonstration of an olfactory bulb-brain translocation pathway for ZnO nanoparticles in rodent cells in vitro and in vivo |
| Authors | Kao, YY; Cheng, TJ; Yang, DM; Wang, CT; Chiung, YM; Liu, PS |
| Journal | Journal of Molecular Neuroscience |
| Volume | 48 |
| Issue | 2 |
| Page Numbers | 464-471 |
| Abstract | ZnO nanoparticles (ZnO-NPs) are widely used in the engineering and cosmetic industries, and inhaled airborne particles pose a known hazard to human health; their translocation into humans is a recognized public health concern. The pulmonary-blood pathway for ZnO-NP toxicity is well documented, but whether translocation of these particles can also occur via an olfactory bulb-brain route remains unclear. The potential toxicity of ZnO-NPs for the human central nervous system (CNS) is predicated on the possibility of their translocation. Our study investigated translocation of ZnO-NPs both in vitro using the neuronal cell line PC12 and in vivo in a Sprague-Dawley rat model. Our findings indicate that the zinc-binding dye, Newport-Green DCF, binds ZnO stoichiometrically and that ZnO-NP concentration can therefore be measured by the fluorescence intensity of the bound dye in confocal fluorescence microscopy. Confocal data obtained using Newport-Green DCF-2 K(+)-conjugated ZnO-NPs along with the membrane probe FM1-43 demonstrated endocytosis of ZnO-NPs by PC12 cells. In addition, Fluozin-3 measurement showed elevation of cytosolic Zn(2+) concentration in these cells. Following in vivo nasal exposure of rats to airborne ZnO-NPs, olfactory bulbs and brains that were examined by Newport-Green fluorescence and TEM particle measurement clearly showed the presence of ZnO-NPs in brain. We conclude that an olfactory bulb-brain translocation pathway for airborne ZnO-NPs exists in rats, and that endocytosis is required for interneuron translocation of these particles. |
| Doi | 10.1007/s12031-012-9756-y |
| Pmid | 22528453 |
| Wosid | WOS:000308958700022 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Beijing, China |
| Conference Name | 16th International Symposium on Chromaffin Cell Biology (ISCCB) |
| Conference Date | July 11-15, 2011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | zinc oxide nanoparticles; Zn2+; PC12 cells; olfactory bulb-brain pathway |