Airborne organic particulate matter (PM) activates phospholipase A2 through extracellular signal-regulated kinase in lung epithelial cells
Parinandi, NL; Kotha, SR
HERO ID
2579704
Reference Type
Journal Article
Subtype
Abstract
Year
2008
Language
English
| HERO ID | 2579704 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2008 |
| Title | Airborne organic particulate matter (PM) activates phospholipase A2 through extracellular signal-regulated kinase in lung epithelial cells |
| Authors | Parinandi, NL; Kotha, SR |
| Journal | Free Radical Biology and Medicine |
| Volume | 45 |
| Issue | Suppl. |
| Page Numbers | S47-S47 |
| Abstract | The precise mechanism(s) of poultry (agricultural organic) particulate matter (PM)-induced respiratory disorders is not established. Hence, it is hypothesized here that poultry PM induces the release of the calcium-dependent phospholipase A2 (cPLA2) by lung epithelium under the modulation of extracellular signal-regulated kinase (ERK) through serine phosphorylation. To test this hypothesis, human lung epithelial cells (A549) in culture, were treated with poultry PM (0.1-1.0 mg) for 0-60 min following which PLA2 activity, cPLA2 serine phosphorylation, and ERK1/2 phosphorylation (activation) were determined. the results showed a significant activation of PLA2 induced by poultry PM (1.0 mg/mL) at 1 h which was significantly attenuated by AACOCF3 (cPLA2 inhibitor) and PD98057 (ERK1/2 upstream inhibitor), suggesting the modulation of cPLA2 activity by ERK1/2 in cells. ERK1/2 phosphorylation was also induced by poultry PM which paralleled with cPLA2 serine phosphorylation. cPLA2 immunoprecipitates from poultry PM-treated cells exhibited enhanced association of ERK1/2 and cPLA2 in a time-dependent fashion. ERK1/2 inhibition with PD98057 also attenuated the poultry PM-induced serine phosphorylation of cPLA2 in cells. Furthermore, the poultry PM-induced cPLA2 serine phosphorylation was significantly inhibited by PD98057 and by transfection with dominant-negative ERK1/2 DNA in cells. for the first time, this study demonstrated that the agricultural PM induced cPLA2 activation through ERK-mediated serine phosphorylation. |
| Wosid | WOS:000260867900129 |
| Url | http://www.sciencedirect.com/science/article/pii/S0891584908006217 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Indianapolis, IN |
| Conference Name | Society for Free Radical Biology and Medicine 15th Annual Meeting |
| Conference Date | November 19-23, 2008 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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