Blood SC5b-9 complement levels increase at parturition during term and preterm labor
Segura-Cervantes, E; Mancilla-Ramirez, J; Zurita, L; Paredes, Y; Arredondo, JL; Galindo-Sevilla, N
| HERO ID | 2902114 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Blood SC5b-9 complement levels increase at parturition during term and preterm labor |
| Authors | Segura-Cervantes, E; Mancilla-Ramirez, J; Zurita, L; Paredes, Y; Arredondo, JL; Galindo-Sevilla, N |
| Journal | Journal of Reproductive Immunology |
| Volume | 109 |
| Page Numbers | 24-30 |
| Abstract | We explored the hypothesis that complement, an innate and adaptive immune effector, is active in the plasma of parturient women and is deposited on fetal membranes collected after delivery. A cross-sectional study was designed to evaluate complement activity at parturition. Pregnant women (n=97) between 15 and 41 years of age were enrolled in a hospital protocol during the perinatal period to assess both SC5b-9 complement activity in blood and complement deposition on fetal membranes during parturition. Soluble SC5b-9 complement activity in plasma fractions was measured using a standard enzyme-linked immunosorbent assay (ELISA) that included specific anti-complement antibodies. Complement deposition on membranes was analyzed using immuno-dot blots and immunohistochemistry. Soluble SC5b-9 complement complex levels were increased in the plasma of women during term labor (TL; median 3361; range 1726-5670ng/mL), preterm labor (PL; median 2958; range 1552-7092ng/mL), and preterm premature rupture of membranes (PPROM; median 2272; range 167-6540ng/mL) compared with pregnant women who were not in labor (P; median 1384; range 174-4570ng/mL; P<0.001, Kruskal-Wallis test). Active complement, as assessed by the C9 neo-antigen in C5b-9 complexes, was deposited on fetal membranes, with no difference between term and preterm delivery. The deposition of active complement on fetal membranes was confirmed by immunohistochemistry. Women who underwent non-labor-indicated Cesarean sections did not exhibit complement deposition. Soluble SC5b-9 complement complex levels increased in the plasma of women during parturition, and complement C5b-9 complexes were deposited on fetal membranes. |
| Doi | 10.1016/j.jri.2015.02.008 |
| Pmid | 25868739 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |