Separation behavior of octadecadienoic acid isomers and identification of cis- and trans-isomers using gas chromatography
Shibamoto, S; Gooley, A; Yamamoto, K
HERO ID
2902339
Reference Type
Journal Article
Year
2015
Language
English
PMID
| HERO ID | 2902339 |
|---|---|
| In Press | No |
| Year | 2015 |
| Title | Separation behavior of octadecadienoic acid isomers and identification of cis- and trans-isomers using gas chromatography |
| Authors | Shibamoto, S; Gooley, A; Yamamoto, K |
| Journal | Lipids |
| Volume | 50 |
| Issue | 1 |
| Page Numbers | 85-100 |
| Abstract | Using a strongly polar cyanopropyl capillary column we have investigated the gas chromatography (GC) separation behaviors of 24 octadecadienoic acid methyl ester (18:2ME) isomers compared against saturated methyl stearate (18:0ME) and arachidic acid methyl ester (20:0ME), and the dependency on the GC column temperature. The 24 isomers were obtained by performing cis-to trans-isomerization of six regioisomers: five of the 18:2ME isomers were prepared by the partial reduction of methyl α-linolenate and methyl γ-linolenate C18 trienoic acids with different double bond positions, whereas the sixth isomer, 18:2ME (c5, c9), was obtained from a raw constituent fatty acid methyl ester (FAME) sample extracted from Japanese yew seeds. There are no reference standards commercially available for 18:2ME isomers, and in elucidating the elution order of these isomers this study should help the future identification of cis- and trans-type of 18:2ME. We also report the identification method of cis- and trans-type of FAME using equivalent chain lengths and attempt the identification of cis- and trans-type of 18:2ME isomers from partially hydrogenated canola oil. |
| Doi | 10.1007/s11745-014-3966-8 |
| Pmid | 25466915 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |