Impact of RNA-guided technologies for target identification and deconvolution
Fennell, M; Xiang, Q; Hwang, A; Chen, C; Huang, CH; Chen, CC; Pelossof, R; Garippa, RJ
HERO ID
2902451
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2902451 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Impact of RNA-guided technologies for target identification and deconvolution |
| Authors | Fennell, M; Xiang, Q; Hwang, A; Chen, C; Huang, CH; Chen, CC; Pelossof, R; Garippa, RJ |
| Journal | Journal of Biomolecular Screening |
| Volume | 19 |
| Issue | 10 |
| Page Numbers | 1327-1337 |
| Abstract | For well over a decade, RNA interference (RNAi) has provided a powerful tool for investigators to query specific gene targets in an easily modulated loss-of-function setting, both in vitro and in vivo. Hundreds of publications have demonstrated the utility of RNAi in arrayed and pooled-based formats, in a wide variety of cell-based systems, including clonal, stem, transformed, and primary cells. Over the years, there have been significant improvements in the design of target-specific small-interfering RNA (siRNA) and short-hairpin RNA (shRNA), expression vectors, methods for mitigating off-target effects, and accurately interpreting screening results. Recent developments in RNAi technology include the Sensor assay, high-efficiency miR-E shRNAs, improved shRNA virus production with Pasha (DRGC8) knockdown, and assessment of RNAi off-target effects by using the C9-11 method. An exciting addition to the arsenal of RNA-mediated gene modulation is the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas) system for genomic editing, allowing for gene functional knockout rather than knockdown. |
| Doi | 10.1177/1087057114548414 |
| Pmid | 25163683 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |