Degradation of phenanthrene by the rhizobacterium Ensifer meliloti

Muratova, A; Pozdnyakova, N; Makarov, O; Baboshin, M; Baskunov, B; Myasoedova, N; Golovleva, L; Turkovskaya, O

HERO ID

2902572

Reference Type

Journal Article

Year

2014

Language

English

PMID

25052918

HERO ID 2902572
In Press No
Year 2014
Title Degradation of phenanthrene by the rhizobacterium Ensifer meliloti
Authors Muratova, A; Pozdnyakova, N; Makarov, O; Baboshin, M; Baskunov, B; Myasoedova, N; Golovleva, L; Turkovskaya, O
Journal Biodegradation
Volume 25
Issue 6
Page Numbers 787-795
Abstract The biodegradation of the polycyclic aromatic hydrocarbon phenantherene by the rhizobacterial strain Ensifer meliloti P221, isolated from the root zone of plant grown in PAH-contaminated soil was studied. Bacterial growth and phenanthrene degradation under the influence of root-exuded organic acids were also investigated. Analysis of the metabolites produced by the strain by using thin-layer chromatography, gas chromatography, high-pressure liquid chromatography, and mass-spectrometry revealed that phenanthrene is bioconverted via two parallel pathways. The first, major pathway is through terminal aromatic ring cleavage (presumably at the C3-C4 bond) producing benzocoumarin and 1-hydroxy-2-naphthoic acid, whose further degradation with the formation of salicylic acid is difficult or is very slow. The second pathway is through the oxidation of the central aromatic ring at the C9-C10 bond, producing 9,10-dihydro-9,10-dihydroxyphenanthrene, 9,10-phenanthrenequinone, and 2,2'-diphenic acid. This is the first time that the dioxygenation of phenanthrene at the C9 and C10 atoms, proven by identification of characteristic metabolites, has been reported for a bacterium of the Ensifer genus.
Doi 10.1007/s10532-014-9699-9
Pmid 25052918
Wosid WOS:000342174000002
Is Certified Translation No
Dupe Override No
Comments Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907071745&doi=10.1007%2fs10532-014-9699-9&partnerID=40&md5=34e084bc0d185481fe9185a848cc2a35
Is Public Yes
Language Text English
Keyword Biodegradation pathways; Ensifer meliloti; Phenanthrene