Mapping of the complement C9 binding domain on Trichinella spiralis paramyosin
Zhao, X; Hao, Y; Yang, J; Gu, Y; Zhu, X
HERO ID
2902809
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2902809 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Mapping of the complement C9 binding domain on Trichinella spiralis paramyosin |
| Authors | Zhao, X; Hao, Y; Yang, J; Gu, Y; Zhu, X |
| Journal | Parasites & Vectors |
| Volume | 7 |
| Page Numbers | 80 |
| Abstract | <strong>BACKGROUND: </strong>Trichinellosis is an important foodborne zoonosis that is distributed worldwide. Trichinella spiralis may evade host complement-mediated attack by expressing complement inhibitory proteins, such as paramyosin (Pmy). Previous studies have shown that Trichinella spiralis paramyosin (Ts-Pmy) is able to bind to the human complement component C9 to inhibit the complement activation and protect the parasite from complement-mediated attack. Further determination of the complement-binding domain on Ts-pmy will enable us to better understand the Ts-Pmy's biofunction in the immune evasion and provide feasible approach to develop epitope-based subunit vaccine against trichinellosis.<br /><br /><strong>METHODS: </strong>The complement C9 binding region on Ts-Pmy was determined by expression of overlapped fragments of Ts-Pmy and their binding activities to C9. The exact binding site was further narrowed-down to a 14-amino acid peptide at C-terminus using synthesized peptides with different size of amino acid sequence. The C9 complement-binding of the 14-amino acid peptide and its interference in the C9 polymerization and the complement-mediated lysis of rabbit erythrocytes was investigated.<br /><br /><strong>RESULTS: </strong>The protein interaction between human C9 and native Ts-Pmy was further confirmed by immunoprecipitation with T. spiralis lysates. The fragmental expression and C9 binding assays identified that the binding region of Ts-Pmy to C9 is located within 831-885 of Ts-Pmy C-terminus. The exact binding site on Ts-Pmy to C9 was narrowed down to 14 amino acid residues (⁸⁶⁶Val-⁸⁷⁹Met) by using different sizes of synthesized peptides. In the presence of the synthesized 14-amino acid peptide, human C9 polymerization and the hemolytic activity of the human complement was inhibited.<br /><br /><strong>CONCLUSIONS: </strong>Our results revealed the precise molecular basis for T. spiralis to produce Ts-Pmy as an immunomodulator to evade the attack of the host complement system as a survival mechanism. |
| Doi | 10.1186/1756-3305-7-80 |
| Pmid | 24564979 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |