Positive effects of PETN on endothelial function in hypertensive rats
Oelze, M; Schuhmacher, S; Wenzel, P; Jansen, T; Stalleicken, D; Maethner, F; Kamuf, J; Sydow, L; Muenzel, T; Daiber, A
HERO ID
3078318
Reference Type
Journal Article
Subtype
Abstract
Year
2008
Language
English
| HERO ID | 3078318 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2008 |
| Title | Positive effects of PETN on endothelial function in hypertensive rats |
| Authors | Oelze, M; Schuhmacher, S; Wenzel, P; Jansen, T; Stalleicken, D; Maethner, F; Kamuf, J; Sydow, L; Muenzel, T; Daiber, A |
| Journal | Free Radical Biology and Medicine |
| Volume | 45 |
| Issue | Suppl. |
| Page Numbers | S148-S148 |
| Abstract | In contrast to other organic nitrates pentaerithrityl tetranitrate (PETN)-treatment in Wistar rats induces neither nitrate tolerance nor cross-tolerance, which can be explained by induction of antioxidant mechanisms like heme oxygenase-1 (HO-1) and Ferritin. With the present studies we tested in an animal model of angiotensin-II (AT-II) induced hypertension, whether chronic treatment with PETN will preserve its beneficial effects compared to the effects of chronic treatment with Isosorbitmononitrate (ISMN). Therefore male wistar rats (230-250 g) were treated with AT-II (1 mg/kg/d) alone or together with PETN (15 mg/kg/d) or ISMN (75 mg/kg/d) via subcutaneous osmotic minipumps for 7 days. Control pumps were applied with the solving reagent, respectively. Ang-II infusion caused endothelial dysfunction and reduced endothelial independent relaxation, indicated by a decreased vasodilator potency of acetylcholine and nitroglycerin in isolated aortic rings, an increase of reactive superoxide species (ROS) in aortic vessels and in NADPH oxidase activity in heart membrane fractions, as detected by lucigenin- (5µM) derived chemiluminescence (LDCL). Staining of aortic sections and HPLC-derived measurements with the fluorescent dye dihydroethidine showed O2− formation throughout the vessel wall. In addition, expression of HO-1 was increased in aorta. in contrast to ISMN, co-treatment with PETN normalized in part vascular function and ROS-formation. Furthermore, HO-1 expression was further enhanced by PETN, not by ISMN. Thus, the beneficial effects of PETN on AT-II induced hypertension may be also explained by induction of antioxidant mechanisms and thereby its preserved nitrovasodilatory action. |
| Wosid | WOS:000260867900418 |
| Url | http://www.sciencedirect.com/science/article/pii/S0891584908006321 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Indianapolis, IN |
| Conference Name | Society for Free Radical Biology and Medicine 15th Annual Meeting |
| Conference Date | November 19-23, 2008 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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