Conversion of naphthalene to trans-naphthalene dihydrodiol: evidence for the presence of a coupled aryl monooxygenase-epoxide hydrase system in hepatic microsomes
Oesch, F; Daly, J
HERO ID
32853
Reference Type
Journal Article
Year
1972
Language
English
PMID
| HERO ID | 32853 |
|---|---|
| In Press | No |
| Year | 1972 |
| Title | Conversion of naphthalene to trans-naphthalene dihydrodiol: evidence for the presence of a coupled aryl monooxygenase-epoxide hydrase system in hepatic microsomes |
| Authors | Oesch, F; Daly, J |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 46 |
| Issue | 4 |
| Page Numbers | 1713-1720 |
| Abstract | Trichloropropene oxide (TCPO), a potent uncompetitive inhibitor of microsomal epoxide hydrases, reacts rapidly and stoichiometrically with glutathione. Thus, in liver preparations containing glutathione, TCPO enhances dihydrodiol formation from naphthalene by preventing a competing reaction of the intermediate naphthalene oxide with glutathione. In guinea pig liver preparations, in which glutathione has been removed by titration with diethyl maleate, TCPO now effectively inhibited hydration of naphthalene oxide, but only partially inhibited formation of dihydrodiol from naphthalene. This and other evidence suggest that a coupled monooxygenase-hydrase system, inducible by 3-methylcholanthrene, is present in liver microsomes. The resistance of the system in guinea pig liver to inhibition by TCPO is readily lost during freezing and thawing and upon resuspension of sedimented microsomes. Such a coupled system may convert aromatic compounds rapidly to dihydrodiols, thereby circumventing toxic effects of intermediate arene oxides. © 1972. |
| Doi | 10.1016/0006-291X(72)90807-8 |
| Pmid | 5015607 |
| Wosid | WOS:A1972L798500035 |
| Url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0015522565&doi=10.1016%2f0006-291X%2872%2990807-8&partnerID=40&md5=c60f97d8cdf08c846ae0552148507478 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | trans is underlined.Biochem. Biophys. Res. Commun. 46: 1713-1720.Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0015522565&doi=10.1016%2f0006-291X%2872%2990807-8&partnerID=40&md5=c60f97d8cdf08c846ae0552148507478 |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |
| Relationship(s) |
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