Simultaneous determination of carbonyl compounds and polycyclic aromatic hydrocarbons in atmospheric particulate matter by liquid chromatography-diode array detection-fluorescence detection
Prieto-Blanco, MC; Iglesias, MP; Lopez-Mahia, P; Lorenzo, SM; Rodriguez, DP
HERO ID
385199
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 385199 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Simultaneous determination of carbonyl compounds and polycyclic aromatic hydrocarbons in atmospheric particulate matter by liquid chromatography-diode array detection-fluorescence detection |
| Authors | Prieto-Blanco, MC; Iglesias, MP; Lopez-Mahia, P; Lorenzo, SM; Rodriguez, DP |
| Journal | Talanta |
| Volume | 80 |
| Issue | 5 |
| Page Numbers | 2083-2092 |
| Abstract | Simultaneous analysis of 24 carbonyl compounds (alkanals, unsaturated, dicarbonylic and aromatic aldehydes and ketones) derivatized with 2,4-dinitrophenylhydrazine and 16 polycyclic aromatic hydrocarbons (PAHs) using a photodiode-array (PDA) and a fluorescence (FL) detector in series is proposed. The separation is carried out with a reversed-phase column and gradient elution using four solvents (acetonitrile, water, tetrahydrofuran and methanol) in less than 35 min. Several critical pairs of carbonyl compounds with 3 and 4 carbon atoms and different functional groups, isomers of tolualdehyde, aromatic and aliphatic aldehydes were conditional on the gradient elution. Common pre-treatment for two groups of compounds consists in a step of extraction and derivatization in aqueous medium and a further clean-up using a polymeric phase SPE and concentration in a mixture of dichloromethane:methanol. A pre-concentration factor of 50 was achieved by this procedure. Acetone and formaldehyde blanks were minimized and remain controlled with a specific cleaning of glass material and washing the SPE cartridge. The limits of detection (LOD) ranged from 0.006 to 0.18 ng mL(-1) for PAHs and from 2.4 to 10.1 ng mL(-1) for carbonyl compounds and method precision was <or=15% for all analysed compounds. Recoveries were within the range of 95-104% for PAHs except for more volatile compounds (acenaphthene and fluorene) and within the range of 72-113% for carbonyl compounds. The method was applied in water-soluble fraction of PM(10) (atmospheric particulate matter with an aerodynamic diameter less than 10 microm) and the spectral contrast technique was used in the identification of carbonyl compounds. |
| Doi | 10.1016/j.talanta.2009.11.009 |
| Pmid | 20152456 |
| Wosid | WOS:000275612500075 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000275612500075 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Carbonyl compounds; Polycyclic aromatic hydrocarbons; HPLC-PDA-FL; Simultaneous analysis; PM10; Water-soluble organic compounds (WSOC) |
| Is Qa | No |