Metabolic fate of an oral dose of 15-N-labeled nitrate in humans: Effect of diet supplementation with ascorbic acid
Wagner, DA; Schultz, DS; Deen, WM; Young, VR; Tannenbaum, SR
HERO ID
4138591
Reference Type
Journal Article
Year
1983
Language
English
PMID
| HERO ID | 4138591 |
|---|---|
| In Press | No |
| Year | 1983 |
| Title | Metabolic fate of an oral dose of 15-N-labeled nitrate in humans: Effect of diet supplementation with ascorbic acid |
| Authors | Wagner, DA; Schultz, DS; Deen, WM; Young, VR; Tannenbaum, SR |
| Journal | Cancer Research |
| Volume | 43 |
| Issue | 4 |
| Page Numbers | 1921-1925 |
| Abstract | The metabolic fate of a p.o. dose of 3.5 mmol 15N-labeled nitrate has been investigated in 12 healthy young adults. Samples of urine, saliva, plasma, and feces were collected over a period of 48 hr following administration of the dose. Subjects received either 60 mg of ascorbic acid, 2 g of ascorbic acid, or 2 g of sodium ascorbate per day. An average of 60% of the 15NO3- dose appeared in the urine as nitrate within 48 hr. Less than 0.1% appeared in the feces. The 15N label of nitrate was also found in the urine (3%) and feces (0.2%) in the form of ammonia or urea. The fate of the remaining 35% of the 15NO3- dose administered is unknown. No effect of ascorbic acid or sodium ascorbate on the nitrate and nitrite levels of plasma, saliva, urine, or feces was observed. A one-compartment pharmacokinetic model was used to describe the relationships between intake, plasma concentration, and urinary excretion of nitrate. The half-life of nitrate in the body was found to be approximately 5 hr, and its volume of distribution was about 30% of body weight. Daily endogenous biosynthesis of nitrate was estimated to be about 1 mmol/day. |
| Pmid | 6831427 |
| Wosid | WOS:A1983QK10100075 |
| Url | https://aacrjournals.org/cancerres/article/43/4/1921/487304/Metabolic-Fate-of-an-Oral-Dose-of-15N-labeled |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |