A glucose biosensor based on the polymerization of aniline induced by a bio-interphase of glucose oxidase and horseradish peroxidase
Gong, C; Chen, J; Song, Y; Sun, Min; Song, Y; Guo, Q; Wang, Li
| HERO ID | 4339956 |
|---|---|
| In Press | No |
| Year | 2016 |
| Title | A glucose biosensor based on the polymerization of aniline induced by a bio-interphase of glucose oxidase and horseradish peroxidase |
| Authors | Gong, C; Chen, J; Song, Y; Sun, Min; Song, Y; Guo, Q; Wang, Li |
| Journal | Analytical Methods |
| Volume | 8 |
| Issue | 7 |
| Page Numbers | 1513-1519 |
| Abstract | A novel glucose biosensor was developed based on a 4-amino thiophenol/Au nanoparticle/glucose oxidase (GOD)-horseradish peroxidase (HRP)/6-mercapto-1-hexanol-11-mercaptoundecanoic acid/Au electrode. The modified electrode could be used to detect glucose based on the polymerization of aniline induced by HRP and H2O2 which was produced by the reduction of O-2 accompanied by the oxidation of glucose into gluconic acid via GOD. With the increase of glucose, more H2O2 molecules were produced and then a growing number of polyaniline (PANI) molecules were formed on the modified electrode surface, which resulted in the decrease of peak current of Fe(CN)(6)(3-/4-) because the formed PANI hindered the electron transfer of Fe(CN) 6(3-/4-) toward the electrode surface. Based on the decrease of peak current of Fe(CN)(6)(3-/4-), the glucose concentration could be determined accurately and selectively. The resulted biosensor exhibited a wide linear range from 16.5 mM to 10.0 mM, a high sensitivity of 41.78 mu A mM(-1) cm(-2) and good selectivity. The glucose biosensor exhibits some advantages over the traditional GOD electrodes in terms of a close to zero applied potential. Moreover, the used electrode might be used as a H2O2 sensor due to its good electrocatalytic response for H2O2. |
| Doi | 10.1039/c5ay02762d |
| Wosid | WOS:000370757000009 |
| Url | <Go to ISI>://WOS:000370757000009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | article; analytical methods; aniline; biosensors; electrodes; electron transfer; ferricyanides; ferrocyanides; gluconic acid; glucose; glucose oxidase; hydrogen peroxide; nanogold; oxidation; peroxidase; polymerization; polymers |