Catabolism of D-glucose by Pseudomonas putida U occurs via extracellular transformation into D-gluconic acid and induction of a specific gluconate transport system
Schleissner, C; Reglero, A; Luengo, JM
| HERO ID | 4940979 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Catabolism of D-glucose by Pseudomonas putida U occurs via extracellular transformation into D-gluconic acid and induction of a specific gluconate transport system |
| Authors | Schleissner, C; Reglero, A; Luengo, JM |
| Journal | Microbiology (Reading, England) |
| Volume | 143 ( Pt 5) |
| Page Numbers | 1595-1603 |
| Abstract | Pseudomonas putida U does not degrade D-glucose through the glycolytic pathway but requires (i) its oxidation to D-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of D-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of D-gluconic acid. This uptake system showed maximal rates of transport at 30 degrees C in 50 mM potassium phosphate buffer, pH 7.0. Under these conditions the K(m) calculated for D-gluconic acid was 6.7 microM. Furthermore, a different transport system, specific for the uptake of glucose, was also identified. It is active and shows maximal uptake rates at 35 degrees C in 50 mM potassium phosphate buffer, pH 6.0, with a K(m) value of 8.3 microM. |
| Doi | 10.1099/00221287-143-5-1595 |
| Pmid | 9168611 |
| Wosid | WOS:A1997WZ94400017 |
| Is Certified Translation | No |
| Dupe Override | 4940979 |
| Is Public | Yes |
| Language Text | English |