The athymic nude rat: III. Natural cell-mediated cytotoxicity
de Jong, WH; Steerenberg, PA; Ursem, PS; Osterhaus, ADME; Vos, JG; Ruitenberg, EJ
HERO ID
5011200
Reference Type
Journal Article
Year
1980
Language
English
PMID
| HERO ID | 5011200 |
|---|---|
| In Press | No |
| Year | 1980 |
| Title | The athymic nude rat: III. Natural cell-mediated cytotoxicity |
| Authors | de Jong, WH; Steerenberg, PA; Ursem, PS; Osterhaus, ADME; Vos, JG; Ruitenberg, EJ |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 17 |
| Issue | 2 |
| Page Numbers | 163-172 |
| Abstract | Homozygous rnu/rnu and heterozygous +/rnu rats were investigated and compared with each other for the existence of natural cell-mediated cytotoxicity. Investigated were total, adherent and nonadherent cell populations from spleen, peritoneal cavity and mesenteric lymph node. The natural killer (NK) cell activity was measured in a 4 h 51Cr-release assay with a xenogeneic murine YAC lymphoma target cell line. In rnu/rnu and +/rnu rats the peritoneal cavity had the highest percentage of activity; the spleen and mesenteric lymph node showed a lower activity. The mesenteric lymph node of +/rnu rats 8-10 wk of age expressed a very low activity, in contrast to a very high activity in rnu/rnu rats. For almost every effector to target cell (E:T) ratio investigated (100, 70, 50 and 10), the natural killer cell activity in the nude rats was significantly higher than in their thymus-bearing littermates. In comparison with that of +/rnu rats, NK activity in the nonadherent cell fraction of athymic rats was 50 to 60% higher in spleen cells, doubled in peritoneal cells and increased 10-fold or higher in lymph node cells. Investigations of rat sera for the presence of antibodies against frequently occurring rodent viruses were negative. |
| Doi | 10.1016/0090-1229(80)90084-7 |
| Pmid | 7408240 |
| Wosid | BCI:BCI198171010373 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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