Disruption of microtubule assembly and spindle formation as a mechanism for the induction of aneuploid cells by sodium arsenite and vanadium pentoxide
Ramírez, P; Eastmond, DA; Laclette, JP; Ostrosky-Wegman, P
HERO ID
594587
Reference Type
Journal Article
Year
1997
Language
English
PMID
| HERO ID | 594587 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Disruption of microtubule assembly and spindle formation as a mechanism for the induction of aneuploid cells by sodium arsenite and vanadium pentoxide |
| Authors | Ramírez, P; Eastmond, DA; Laclette, JP; Ostrosky-Wegman, P |
| Journal | Mutation Research |
| Volume | 386 |
| Issue | 3 |
| Page Numbers | 291-298 |
| Abstract | Arsenic and vanadium are important environmental and industrial pollutants. Due to their widespread occurrence and potential genotoxicity, we studied the aneuploidy-inducing effects of these elements in cultured human lymphocytes using a variety of techniques including fluorescence in situ hybridization (FISH) with DNA probes for chromosomes 1 and 7, immunostaining of the lymphocyte spindle apparatus, and an in vitro assay measuring the polymerization and depolymerization of tubulin. Dose-related increases in hyperdiploidy were seen in lymphocyte cultures treated with sodium arsenite (NaAsO2) or vanadium pentoxide (V2O5) over concentrations ranging from 0.001 to 0.1 microM. NaAsO2-treated cells from different donors exhibited similar hyperdiploid frequencies, whereas substantial inter-individual variability was seen in the V2O5-treated cells. Examination of the spindle apparatus using an anti-beta-tubulin antibody indicated that these compounds might disrupt spindle formation by interacting with microtubules. Additional in vitro assays using purified tubulin indicated that both compounds inhibited microtubule assembly and induced tubulin depolymerization. These results indicate that in vitro exposure to both NaAsO2 and V2O5 can induce aneuploidy in human lymphocytes, and that this effect may occur through a disruption of microtubule function. |
| Doi | 10.1016/S1383-5742(97)00018-5 |
| Pmid | 9219566 |
| Wosid | WOS:A1997XG03500011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Authoring Organization: International Programme on Chemical Safety (IPCS)|WOS:A1997XG03500011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>DCN-240008</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Arsenic compounds</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Vanadium compounds</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Blood cells</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Humans</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>In vitro studies</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Genotoxic effects</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Dose response</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chromosome disorders</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Cellular structures</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Cell damage</kw> |
| Is Qa | No |