Induction of PDGF receptor-α in rat myofibroblasts during pulmonary fibrogenesis in vivo
Bonner, JC; Lindroos, PM; Rice, AB; Moomaw, CR; Morgan, DL
HERO ID
595437
Reference Type
Journal Article
Year
1998
Language
English
PMID
| HERO ID | 595437 |
|---|---|
| In Press | No |
| Year | 1998 |
| Title | Induction of PDGF receptor-α in rat myofibroblasts during pulmonary fibrogenesis in vivo |
| Authors | Bonner, JC; Lindroos, PM; Rice, AB; Moomaw, CR; Morgan, DL |
| Journal | American Journal of Physiology |
| Volume | 274 |
| Issue | 1 |
| Page Numbers | L72-L80 |
| Abstract | Platelet-derived growth factor (PDGF) is a potent mitogen for mesenchymal cells. Induction of the PDGF receptor-alpha (PDGF-R alpha) in vitro enhances PDGF-induced mitogenesis and chemotaxis. Thus we investigated whether the PDGF-R alpha is induced in vivo during pulmonary fibrogenesis using a vanadium pentoxide (V2O5) model of lung injury. PDGF-R alpha mRNA expression was induced 24 h postinstillation. PDGF-R beta mRNA was constitutively expressed and did not increase. Western blotting showed upregulation of PDGF-R alpha protein by 48 h, and immunohistochemical analysis localized PDGF-R alpha primarily in mesenchymal cells residing within fibrotic lesions. Upregulation of PDGF-R alpha in vivo preceded mesenchymal cell hyperplasia (3-7 days) and collagen deposition by day 15. Supernatants from alveolar macrophages treated with V2O5 in vitro released upregulatory activity for PDGF-R alpha on cultured lung myofibroblasts, and this activity was blocked by the interleukin-1-receptor antagonist. These data suggest that interleukin-1 beta-mediated induction of PDGF-R alpha in vivo is important to lung myofibroblast hyperplasia during fibrogenesis. |
| Doi | 10.1152/ajplung.1998.274.1.L72 |
| Pmid | 9458803 |
| Wosid | WOS:000071572600010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | lung fibrosis; vanadium pentoxide; platelet-derived growth factor-receptor system; interleukin-1; transforming growth factor-b1 |