Exposure to styrene: Comparison of DNA and haemoglobin adducts as biomarkers
Latriano, L; Wazneh, L; Dong, Z; Lu, SJ; Snyder, C; Jeffrey, AM
| HERO ID | 597864 |
|---|---|
| Year | 1991 |
| Title | Exposure to styrene: Comparison of DNA and haemoglobin adducts as biomarkers |
| Book Title | Human carcinogen exposure. Biomonitoring and risk assessment |
| Authors | Latriano, L; Wazneh, L; Dong, Z; Lu, SJ; Snyder, C; Jeffrey, AM |
| Publisher Text | Oxford University Press |
| City | New York, NY |
| Volume | Biomonitoring and Risk Assessment |
| Page Numbers | 305-314 |
| Abstract | The use of phosphorus-32 (P32) postlabeling for the identification of styrene-oxide adducts was discussed and compared with the analysis of hemoglobin adducts. DNA isolated from the lungs, livers, and lymphocytes of male Fischer-rats exposed to 1000 parts per million styrene (100425) vapors for 6 hours/day for 5 days were analyzed as well as calf thymus DNA modified in-vitro with styrene-oxide (96093). Mutagenicity assays were also performed with Salmonella-typhimurium (TA-100) exposed to styrene-oxide at doses up to 16 micromoles/plate. The analysis of DNA using P32 postlabeling and the separation and purification of P32 labeled styrene-oxide/DNA adducts were described. Procedures for the isolation of globin from rat erythrocytes and the subsequent gas chromatographic/mass spectrometric analysis for hemoglobin adducts after a reaction with pentafluorophenylisothiocyanate were performed. Seven adducts were separated following P32 postlabeling and chromatography using five solvents. The limit of detection of the P32 assay was 1 adduct/10(4) nucleotides. The sensitivity of the method was enhanced using both the nuclease-P1 and butanol procedures for DNA adduct enrichment. A mutagenic response was seen following exposure of S-typhimurium to styrene-oxide, with DNA adducts detected only following exposure to 20 micromoles/milliliter or more. Identical adduct patterns were seen in styrene as well as air exposed rats after P32 postlabeling of lung, liver, or lymphocyte DNA. The analysis of hemoglobin adducts yielded similar retention times for the styrene-oxide/valine derivatives. |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Human Carcinogen Exposure ISSN: |
| Is Public | Yes |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>DCN-230018</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Laboratory techniques</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Analytical methods</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>DNA adducts</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>DNA damage</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Organic solvents</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biological monitoring</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chromatographic analysis</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Quantitative analysis</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Radioassays</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>In vitro studies</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Aromatic hydrocarbons</kw> |
| Relationship(s) |
|