Identification and expansion of the tumorigenic lung cancer stem cell population
Eramo, A; Lotti, F; Sette, G; Pilozzi, E; Biffoni, M; Di Virgilio, A; Conticello, C; Ruco, L; Peschle, C; De Maria, R
| HERO ID | 605192 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Identification and expansion of the tumorigenic lung cancer stem cell population |
| Authors | Eramo, A; Lotti, F; Sette, G; Pilozzi, E; Biffoni, M; Di Virgilio, A; Conticello, C; Ruco, L; Peschle, C; De Maria, R |
| Journal | Cell Death and Differentiation |
| Volume | 15 |
| Issue | 3 |
| Page Numbers | 504-514 |
| Abstract | Lung carcinoma is often incurable and remains the leading cancer killer in both men and women. Recent evidence indicates that tumors contain a small population of cancer stem cells that are responsible for tumor maintenance and spreading. The identification of the tumorigenic population that sustains lung cancer may contribute significantly to the development of effective therapies. Here, we found that the tumorigenic cells in small cell and non-small cell lung cancer are a rare population of undifferentiated cells expressing CD133, an antigen present in the cell membrane of normal and cancer-primitive cells of the hematopoietic, neural, endothelial and epithelial lineages. Lung cancer CD133<sup>+</sup> cells were able to grow indefinitely as tumor spheres in serum-free medium containing epidermal growth factor and basic fibroblast growth factor. The injection of 10<sup>4</sup> lung cancer CD133<sup>+</sup> cells in immunocompromised mice readily generated tumor xenografts phenotypically identical to the original tumor. Upon differentiation, lung cancer CD133<sup>+</sup> cells acquired the specific lineage markers, while loosing the tumorigenic potential together with CD133 expression. Thus, lung cancer contains a rare population of CD133<sup>+</sup> cancer stem-like cells able to self-renew and generates an unlimited progeny of non-tumorigenic cells. Molecular and functional characterization of such a tumorigenic population may provide valuable information to be exploited in the clinical setting.Cell Death and Differentiation (2008) 15, 504–514; doi:10.1038/sj.cdd.4402283; published online 30 November 2007 [ABSTRACT FROM AUTHOR] Copyright of Cell Death & Differentiation is the property of Nature Publishing Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | LUNGS -- Cancer; ONCOGENIC DNA viruses; CELL populations; CANCER -- Patients; GENETICS; cancer stem cells; CD133; lung cancer; pulmonary stem cells; stem cell markers; tumor sphere |
| Is Qa | No |