Acetone potentiation of rat liver injury induced by trichloroethylene-carbon tetrachloride mixtures
Charbonneau, M; Oleskevich, S; Brodeur, J; Plaa, GL
| HERO ID | 60772 |
|---|---|
| In Press | No |
| Year | 1986 |
| Title | Acetone potentiation of rat liver injury induced by trichloroethylene-carbon tetrachloride mixtures |
| Authors | Charbonneau, M; Oleskevich, S; Brodeur, J; Plaa, GL |
| Journal | Toxicological Sciences |
| Volume | 6 |
| Issue | 4 |
| Page Numbers | 654-661 |
| Abstract | The effect of acetone (67641) on trichloroethylene (79016) and carbon-tetrachloride (56235) hepatotoxicity was studied in rats. Male Sprague-Dawley-rats were administered 0, 0.25, 0.75, or 1.5 milliliters per kilogram (ml/kg) acetone orally. Eighteen hours later they were injected intraperitoneally with 0 or 0.25ml/kg trichloroethylene or 0.1 or 0.6ml/kg carbon-tetrachloride alone or in combination. The rats were killed 24 hours later, the livers were removed, and assayed for hepatotoxicity by determining plasma alanine-aminotransferase (ALT) activity, total bilirubin, and by histological examination. Acetone alone or combined with trichloroethylene had no effect on ALT activity or total bilirubin. ALT activity was higher in rats treated with trichloroethylene plus either dose of carbon-tetrachloride than with carbon-tetrachloride alone. Bilirubin concentration was increased only by trichloroethylene plus 0.6ml/kg carbon-tetrachloride. Acetone enhanced carbon-tetrachloride liver injury to a greater extent than trichloroethylene. Acetone markedly potentiated liver injury induced by trichloroethylene plus carbon-tetrachloride in a dose/dependent manner. Histological analyses showed no statistical differences in the percentages of normal, degenerated, or necrotic hepatocytes between carbon-tetrachloride or trichloroethylene treated rats and controls. Trichloroethylene plus carbon-tetrachloride significantly reduced the percentage of normal hepatocytes and increased the percentage of degenerated or necrotic hepatocytes. Acetone pretreated rats given the carbon-tetrachloride plus trichloroethylene mixtures showed a significant dose related decrease in the percentage of normal hepatocytes and a dose related increase in the percentage of necrotic hepatocytes. The authors conclude that trichloroethylene can potentiate carbon-tetrachloride induced liver injury. Acetone exerts a potentiating effect on the hepatotoxic response of the carbon-tetrachloride plus trichloroethylene mixtures. |
| Pmid | 3710035 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Fundam. Appl. Toxicol. 6: 654-661. |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |