The role of mouse intestinal microflora in the metabolism of trichloroethylene, an in vivo study
Moghaddam, AP; Abbas, R; Fisher, JW; Lipscomb, JC
HERO ID
630794
Reference Type
Journal Article
Year
1997
Language
English
PMID
| HERO ID | 630794 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | The role of mouse intestinal microflora in the metabolism of trichloroethylene, an in vivo study |
| Authors | Moghaddam, AP; Abbas, R; Fisher, JW; Lipscomb, JC |
| Journal | Human & Experimental Toxicology |
| Volume | 16 |
| Issue | 11 |
| Page Numbers | 629-635 |
| Abstract | 1. Both trichloroethylene and its metabolite, dichloroacetic acid, produce liver tumors peroxisome proliferation and other adverse cellular alterations in rodents. 2. The hepatic mechanism by which dichloroacetic acid is formed is not conclusively demonstrated, but pharmacokinetic models have successfully associated its formation with trichloroacetic acid as immediate precursor. 3. Previous investigations have shown that dichloroacetic acid is formed from trichloroacetic acid by gut microflora isolated in vitro. 4. To determine the impact of gut microflora on dichloroacetic acid formation from a trichloroethylene dose in vivo, we developed a procedure which reduced gut microflora some 3 orders of magnitude below published levels. 5. The administration of trichloroethylene to control mice and to mice whose gut was practically sterile resulted in equivalent concentrations of dichloroacetic acid and other metabolites in blood and liver, but significantly different content of these metabolites in cecum contents. 6. These data indicate that gut microflora contribute minimally, if at all, to the formation of circulating dichloroacetic acid under these conditions |
| Doi | 10.1177/096032719701601101 |
| Pmid | 9426363 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Air; Animals; Bacteria; blood; Cecum; Dichloroacetate; Intestines; Liver; Male; metabolism; Mice; microbiology; Ohio; Research; Solvents; Toxicology; Trichloroacetic Acid; Trichloroethylene |
| Is Qa | No |