Mutational loss of sensitivity to mutacin GS-5 in Streptococcus pyogenes: characterization of a mutant deficient in receptor protein 10
Franker, CK
| HERO ID | 632640 |
|---|---|
| In Press | No |
| Year | 1980 |
| Title | Mutational loss of sensitivity to mutacin GS-5 in Streptococcus pyogenes: characterization of a mutant deficient in receptor protein 10 |
| Authors | Franker, CK |
| Journal | Antimicrobial Agents and Chemotherapy |
| Volume | 17 |
| Issue | 2 |
| Abstract | By means of a stepwise selection procedure, mutants capable of growing in the presence of relatively high multiplicities of a bacteriocin from Streptococcus mutans GS-5 were obtained from a sensitivie strain of Streptococcus pyogenes. Mutacin-neutralizing activity of cell extracts containing receptor protein was examined in one variant that adsorbed 1/6 the amount of bacteriocin adsorbed by the parent strain under conditions equivalent to "saturation." Partially purified receptor protein from both parent and mutant cells neutralized an equivalent amount of bacteriocin on a weight-to-weight basis, indicating that in vitro there was no significant difference in affinity for the mutacin between the respective receptor fractions. Cell extracts from the mutant, solubilized by treatment with trichloroacetic acid, neither neutralized mutacin activity nor interfered with receptor protein-mediated mutacin neutralization in vitro. The mutant phenotype may thus represent a cell surface density of receptor protein which results in the adsorption of sublethal amounts of mutacin. The mutant retained its sensitivity to other mutacins, e.g., those produced by strains LM-7 and BHT of S. mutans, and did not differ from wild-type cells with respect to either detergent sensitivity (sodium lauryl sulfate and Triton X-100) or to inhibition by penicillin, rifampin, bacitracin, erythromycin, and tetracycline. |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | 10; Adsorption; Affinity; Bacterial Proteins/isolation & purification/ metabolism/pharmacology; Bacteriocins; Cell Surface; Cells; IN VITRO; Phenotype; Receptor; Receptors,Drug/ metabolism; Rifampin; Selection; Sodium; Streptococcus pyogenes/drug effects/ genetics/metabolism; Support; Time Factors; Trichloroacetic Acid |
| Is Qa | No |