Free radical generation during δ-Aminolevulinic acid autoxidation: Induction by hemoglobin and connections with porphyrinpathies
Monteiro, HP; Abdalla, DSP; Augusto, O; Bechara, EJH
HERO ID
655790
Reference Type
Journal Article
Year
1989
Language
English
| HERO ID | 655790 |
|---|---|
| In Press | No |
| Year | 1989 |
| Title | Free radical generation during δ-Aminolevulinic acid autoxidation: Induction by hemoglobin and connections with porphyrinpathies |
| Authors | Monteiro, HP; Abdalla, DSP; Augusto, O; Bechara, EJH |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 271 |
| Issue | 1 |
| Page Numbers | 206-216 |
| Abstract | δ-Aminolevulinic acid (ALA), a heme precursor accumulated in acute intermittent porphyria and saturnism, undergoes autoxidation leading to ammonium ion and probably the corresponding α-ketoaldehyde. This reaction is accelerated by addition of oxyhemoglobin (oxyHb) and other iron complexes. OxyHb is concomitantly oxidized to metHb; the apparent second-order rate constant of oxyHb/ALA coupled oxidation is ca. 10 m−1 min−1.1H NMR and uv spectral studies suggest that ALA undergoes enolization before consuming the dissolved oxygen. Spin-trapping experiments demonstrate formation of both the hydroxyl radical and a substrate-derived carbon-centered radical during ALA oxidation. Generation of active oxygen species by ALA might be related to the neuropathy associated to some acquired and inherited porphyrinpathies. |
| Doi | 10.1016/0003-9861(89)90271-3 |
| Wosid | WOS:A1989U366800023 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |